小麦小穗数调控基因WAPO1的单倍型、遗传效应、地理分布及育种利用分析  

Dissection of haplotypes,geographical distribution and breeding utilization of WAPO1 associated with spike development in wheat

作　　者：丁浦洋 周界光 赵聪豪 唐华苹 牟杨 唐力为[2] 邓梅[1] 魏育明[1] 兰秀锦[1] 马建[1] DING Pu-Yang;ZHOU Jie-Guang;ZHAO Cong-Hao;TANG Hua-Ping;MU Yang;TANG Li-Wei;DENG Mei;WEI Yu-Ming;LAN Xiu-Jin;MA Jian(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Panzhihua Academy of Agricultural and Forestry Sciences,Panzhihua 617061,Sichuan,China)

机构地区：[1]四川农业大学小麦研究所,四川成都611130 [2]攀枝花市农林科学研究院,四川攀枝花617061

出　　处：《作物学报》2022年第9期2196-2209,共14页Acta Agronomica Sinica

基　　金：四川省应用基础研究计划项目(2020YJ0140,2021YJ0503);四川省国际合作交流项目(2021YFH0083)资助。

摘　　要：小麦穗部性状直接与产量相关,是极重要的农艺性状。WAPO1编码F-box蛋白,是水稻小穗数发育相关基因APO1在小麦中的直系同源基因。本研究鉴定了WAPO1的优异单倍型,并对其遗传效应、地理分布和育种利用情况进行了鉴定。通过开发的KASP标记对编码区第140位碱基变异SNP(G/T)进行鉴定,并使用前人报道的启动子InDel标记对WAPO1启动子115 bp的插入缺失进行鉴定,在中国地方小麦中进行单倍型鉴定。结果显示,在前人鉴定到的3种单倍型(H1:140^(G)+115^(deletion),H2:140^(T)+115^(insertion),H3:140^(G)+115^(insertion))基础上,鉴定到了新的第4种单倍型(H4:140^(T)+115^(deletion))并证明H2和H4为优异单倍型,能显著提高小麦每穗小穗数。将开发的KASP标记对223份世界小麦、144份中国育成小麦和119份四川小麦进行分型,筛选出优异单倍型并对其在育种中的利用和分布进行了鉴定,结果表明优异单倍型在中国和世界范围都被广泛选育。Wheat is one of the most important crops,and spike is an important trait affecting yield.WAPO1,encoding an F-box protein,is an ortholog of rice APO1 in wheat.In this study,superior haplotypes of WAPO1 were identified and their genetic ef-fects,geographical distribution,and breeding utilization were further analyzed.The SNP(G/T)in F-box region was identified by using the developed KASP marker and 115 bp insertion/deletion of promoter was identified to analyze the haplotypes by the re-ported InDel marker in Chinese wheat landraces.In addition to the previously identified three haplotypes(H1:140^(G)+115^(deletion),H2:140^(T)+115^(insertion),and H3:140^(G)+115^(insertion)),we identified a new and fourth haplotype(H4:140^(T)+115^(deletion))and proved that H2 and H4 were superior haplotypes,which could significantly increase spikelet number per spike in wheat.The KASP marker was also used to identify the superior haplotypes of Chinese wheat varieties/lines,Sichuan wheat varieties and world wheat varieties,and to explore their distribution and breeding utilization.The results reveal that the superior haplotypes are widely selected and bred in China and the world.

关 键 词：WAPO1 每穗小穗数 优异单倍型 地理分布 育种利用 

分 类 号：S512.1[农业科学—作物学]