优质稻“粤农丝苗”白叶枯病抗性遗传分析及主效QTL qBB-11-1的精细定位  

Genetic analysis and fine mapping of a bacterial blight resistance major QTL qBB-11-1 in high-quality rice‘Yuenong Simiao’

作　　者：薛皦 卢东柏[1] 刘维[1] 陆展华 王石光 王晓飞[1] 方志强 何秀英[1] XUE Jiao;LU Dong-Bai;LIU Wei;LU Zhan-Hua;WANG Shi-Guang;WANG Xiao-Fei;FANG Zhi-Qiang;HE Xiu-Ying(Rice Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding,Guangdong 510640,Guangzhou,China)

机构地区：[1]广东省农业科学院水稻研究所/广东省水稻育种新技术重点实验室,广东广州510640

出　　处：《作物学报》2022年第9期2210-2220,共11页Acta Agronomica Sinica

基　　金：中国博士后科学基金项目(2020M682639);科技创新战略专项资金项目(高水平农科院建设)(广东省农业科学院博士后科研专项BZ201909);广东省现代农业产业技术体系建设项目(2021KJ105);广东省重点领域研发计划项目(2020B0202090003);广东省自然科学基金项目(2018A0303130172,2021A1515110411);广州市基础与应用基础项目(202102080417);广东省学科类重点实验室运行项目(2020B1212060047);广东省农业科学院水稻研究所所长基金项目资助。

摘　　要：白叶枯病是对水稻危害最大的细菌性病害,严重危及我国乃至全球粮食安全。挖掘新的抗病基因是改良水稻对白叶枯病抗病性的重要措施。本研究以广东省及华南稻区主栽的优质抗病水稻品种粤农丝苗为材料,利用抗病品种粤农丝苗和感病品种丽江新团黑谷为亲本构建重组自交系(recombinant inbred lines,RILs)及回交群体,进行接种鉴定及基因定位分析。遗传分析表明:粤农丝苗的抗性由不完全显性的白叶枯病抗病基因控制;重组自交系抗病表型结合重测序结果初定位到一个抗性QTL qBB-11-1,位于11号染色体长臂末端;利用片段重叠群分法将qBB-11-1精细定位在InDel标记P89和P54之间,物理距离约为63 kb,区间内包含6个候选基因,且粤农丝苗中的白叶枯病抗性基因可能是未被报道的新基因。这些研究结果对于主栽品种粤农丝苗的抗性基因挖掘与利用将对华南稻区白叶枯病抗性育种具有重要的代表性意义。Bacterial blight is the most devastating bacterial disease to rice,which seriously endangers food security of China and even the world.Mining new resistance genes is an important measure to improve rice resistance to bacterial blight.Yuenong Simiao,was the main rice cultivar with good grain quality and disease resistance in South China.In this study,Yuenong Simiao(YN)and Lijiangxintuanheigu(LTH)were used as parental materials to construct recombinant inbred lines(RILs)and backcross populations.We carried out inoculation identification and gene mapping analysis.Genetic analysis showed that the resistance of YN was controlled by incomplete dominant resistance genes of bacterial blight.Combined the resistance phenotype of recombinant inbred lines with genome resequencing,a resistance QTL qBB-11-1 was identified at the end of the long arm of chromosome 11.Substitution mapping revealed that qBB-11-1 was located between InDel markers P89 and P54 with a physical distance of about 63 kb.There were six candidate genes in this region,and the resistance gene to bacterial blight in YN may be a new gene.It is of great representative significance to explore and utilize the resistance genes of YN in rice breeding for bacterial blight resistance in South China.

关 键 词：水稻 粤农丝苗 白叶枯病 抗病 QTL 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]