对水稻种子耐储性QTL的研究  

QTL mapping of seed storage tolerance in rice(Oryza sativa L.)

作　　者：黄祎雯 孙滨 程灿[2] 牛付安[2] 周继华[2] 张安鹏 涂荣剑[2] 李瑶 姚瑶 代雨婷 谢开珍 陈小荣[1] 曹黎明[2] 储黄伟[2] HUANG Yi-Wen;SUN Bin;CHENG Can;NIU Fu-An;ZHOU Ji-Hua;ZHANG An-Peng;TU Rong-Jian;LI Yao;YAO Yao;DAI Yu-Ting;XIE Kai-Zhen;CHEN Xiao-Rong;CAO Li-Ming;CHU Huang-Wei(School of Agricultural Sciences,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Institute of crop breeding and cultivation,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;College of Fisheries and life,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]上海市农业科学院作物育种栽培研究所,上海201403 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《作物学报》2022年第9期2255-2264,共10页Acta Agronomica Sinica

基　　金：上海市水稻产业技术体系建设项目[沪农科产字(2021)第3号]资助。

摘　　要：稻谷的耐储性在种子生产保存和粮食储备中具有重要的意义。本研究以人工陈化的方法对15个三系杂交稻恢复系品种进行筛选,获得了繁11、繁12、繁31、繁32和繁38五个耐储性较好的品种。选择繁38与粳型恢复系繁26为亲本杂交获得F_(1)代,构建了包含154个株系的双单倍体(double haploid,DH)群体。以2b-RAD简化基因组测序技术对亲本和群体中每个株系进行测序,并构建SNP标记遗传图谱。分析水稻在人工陈化10 d和15 d时与耐储藏相关的QTL。共检测到了6个与稻谷耐储性相关的QTL位点,分布于3号、5号、6号、11号和12号染色体上,LOD值介于3.4509~6.8036之间,可解释6.1575%~12.9979%的表型变异,加性效应在-6.7586%到6.1235%范围内。其中qSI-12位点在陈化10 d和陈化15 d两个条件下均能检测到。qSI-5a和qSI-6这2个位点只在陈化10 d时检测到,而qSI-3、qSI-5b和qSI-11这3个位点只在陈化15 d时检测到。此外,还检测到32对上位性互作位点。这些结果丰富了耐储性品种育种的遗传资源,为进一步精细定位耐储性相关的QTL奠定了基础。Rice seed storability is great significance in seed production and grain storage.In this study,15 three-line hybrid rice restorer varieties were screened by artificial aging method,and 5 varieties with good storage tolerance(namely Fan 11,Fan 12,Fan 31,Fan 32,and Fan 38)were obtained.A Double Haploid(DH)population including 154 lines was constructed using F_(1) derived from the crossing between storage tolerance variety,Fan 38,and japonica restorer line,Fan 26.The parents and DH lines were sequenced using 2bB-RAD Reduced-Representation Genome Sequencing,and a genetic linkage map of SNP marker was constructed.QTLs related to storage tolerance after 10 days and 15 days artificial aging for were analyzed,respectively.A total of 6 QTL loci related to rice storage tolerance were detected on chromosomes 3,5,6,11,and 12,with LOD values ranging from 3.4509 to 6.8036,explaining phenotypic variation of 6.1575%–12.9979%,and the additive effect ranged from-6.7586%to 6.1235%.The qSI-12 locus could be detected under both 10-day and 15-day aging conditions.qSI-5a and qSI-6 were detected only after 10 days of artificial aging,while qSI-3,qSI-5b,and qSI-11 were detected only after 15 days of artificial aging.In addi-tion,32 pairs of epistatic interaction sites were detected.These results enriched the genetic resources for the breeding of storage tolerance varieties,and laid a foundation for further fine mapping of QTLs related to storage tolerance.

关 键 词：水稻 耐储藏 人工陈化 QTL分析 DH群体 

分 类 号：S511[农业科学—作物学]