棉花GhIQM1基因克隆及抗黄萎病功能分析  

Cloning and functional verification of GhIQM1 gene of cotton in response to Verticillium wilt

作　　者：李名江 雷建峰[2] 祖丽皮耶·托合尼亚孜 代培红[1] 刘超[1] 刘晓东[1] LI Ming-Jiang;LEI Jian-Feng;ZULIPIYE·Tuoheniyazi;DAI Pei-Hong;LIU Chao;LIU Xiao-Dong(College of Life Sciences,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;College of Agriculture,Xinjiang Agricultural Univer-sity,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业大学生命科学学院,新疆乌鲁木齐830052 [2]新疆农业大学农学院,新疆乌鲁木齐830052

出　　处：《作物学报》2022年第9期2265-2273,共9页Acta Agronomica Sinica

基　　金：新疆农业大学生物学创新团队项目(ITB202108)资助。

摘　　要：棉花是我国重要的经济作物,而黄萎病(Verticillium wilt)是棉花生产上的主要病害,严重危害棉花产量和纤维品质。本研究通过转录组数据分析筛选出1个与棉花抗病相关、不依赖Ca^(2+)的钙调素结合蛋白基因GhIQM1。该基因受黄萎病菌和水杨酸(SA)诱导表达。利用病毒诱导的基因沉默(virus-induced gene silencing,VIGS)技术研究其在棉花抗黄萎病中的功能发现,抑制GhIQM1基因表达增强了植株对黄萎病的抗性,TRV:GhIQM1植株病情指数、维管束褐化程度、体内病原菌积累量都显著低于TRV:00。qRT-PCR分析表明,抑制GhIQM1表达的植株接种黄萎病后,作为参与水杨酸途径中的3个重要基因NPR1、NPR3和PR5的表达量显著高于对照。以上结果初步表明,GhIQM1基因可能通过抑制SA途径负调控了棉花的黄萎病抗性。Cotton is an important economic crop in China,and Verticillium wilt is the main disease in cotton production,which seriously endangers cotton yield and fiber quality.In this project,GhIQM1,encode a Ca^(2+)-independent calmodulin-binding pro-tein in cotton,was screened out by transcriptome analysis.The gene was up-regulated by Verticillium dahliae and salicylic acid treatment in cotton.The function of GhIQM1 was explored in cotton in response to Verticillium wilt using virus-induced gene silencing(VIGS)technology.The results showed that disease index(DI),the symptoms of Verticillium wilt,the degree of brown-ing of vascular tissue,and the relative fungal biomass pathogen accumulation in vivo in the GhIQM1-silenced plants(TRV:GhIQM1)were significantly lower than those in the TRV:00 cotton plant.The qRT-PCR results revealed that the gene ex-pression level of NPR1,NPR3,and PR5,involved in the salicylic acid pathway,were significantly higher than the control after inoculation of Verticillium dahliae.The above results indicate that the GhIQM1 negatively regulates the resistance of cotton to Verticillium wilt through suppressing SA pathway.

关 键 词：棉花 黄萎病 GhIQM1 基因沉默 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]