Effects of Combined Stress from Salt and Herbicide on Microbial Remediation of Soil Contaminated by n-Hexadecane and Phenanthrene  

作　　者：Liu Bingkun Zuo Shuai Wang Ruirui Dong Kangning Zhang Xiuxia Li Ruyue Hao Boyu Li Jing Yin Linghao Bai Yuhao Song Jiayu Gu Yingying Sun Juan 

机构地区：[1]State Key Laboratory of Petroleum Pollution Control,Beijing 102206 [2]Department of Environment and Safety Engineering,China University of Petroleum,Qingdao 266580 [3]Department of Computer Science and Technology,China University of Petroleum,Qingdao 266580

出　　处：《China Petroleum Processing & Petrochemical Technology》2022年第2期126-137,共12页中国炼油与石油化工（英文版）

基　　金：This study was supported by the Open Project Program of State Key Laboratory of Petroleum Pollution Control(Grant No.PPC2019021);the Fundamental Reseach Funds for the central universities(22CX01004A-6);the CNPC Research Institute of Safety and Environmental Technology and CNPC Technology Innovation Fund Research Project(Grant Nos.2017D-5007-0601 and 2018D-5007-0605);the Research and Promotion Project of key technologies for safety and environmental protection of CNPC(2017D-4013).

摘　　要：In this study,physicochemical,cluster,and high-throughput sequencing analyses were used to investigate the joint effects of salt and herbicide(glyphosate)stress factors on the microbial remediation of soil contaminated by n-hexadecane and phenanthrene.Based on the soil’s physical and chemical properties,differences in microbial quantity and enzyme activities were analyzed among the samples,and how that influenced distribution of soil community structure was focused upon.After 120 days of indoor simulated remediation,the cumulative degradation rate of n-hexadecane decreased by 94.92%(blank control),96.96%(low concentration salt and glyphosate stress group),65.07%(high concentration salt and glyphosate stress group),while that of phenanthrene decreased by 87.33%(blank control),86.25%(low concentration salt and glyphosate stress group),58.45%(high concentration salt and glyphosate stress group).The combined stress of salt and herbicides was capable of lowering the reduction efficiency of organic matter,total nitrogen,and total phosphorus,and also restricting the growth of microorganisms and enzyme activities.Cluster analysis results indicated that the non-stress group was similar to the low-concentration compound stress group during different remedial period,whereas both of those differed starkly from the high-concentration compound stress group.High-throughput sequencing revealed that the dominant soil bacteria phyla shifted from Firmicutes to Actinobacteria within 120 days of remediation.

关 键 词：oil-contaminated soil cluster analysis common quantity sequencing microorganisms salt stress glyphosate stress 

分 类 号：X53[环境科学与工程—环境工程] X171