干蟾皮及其炮制品的指纹图谱建立与化学模式识别  

Establishment of fingerprint and identification of chemical pattern of Bufonis Coriumand processed products

作　　者：周谧 徐丹洋 孙成静 钱洁 ZHOU Mi;XU Dan-yang;SUN Cheng-jing;QIAN Jie(Nantong Food and Drug Control Center,Nantong 226006,China)

机构地区：[1]南通市食品药品监督检验中心,南通226006

出　　处：《药物分析杂志》2022年第5期875-883,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：江苏省南通市科技计划项目(JC2019134)。

摘　　要：目的:建立干蟾皮生品及炮制品UPLC指纹图谱,并同时测定干蟾皮中指标性成分日蟾毒它灵、沙蟾毒精、蟾蜍它里定、蟾毒它灵、华蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基、沙蟾毒精-3-辛二醇辛酯的含量,通过化学模式识别控制干蟾皮质量。方法:干蟾皮的甲醇提取液的分析采用Waters Acquity UPLC BEH色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.5%乙酸为流动相,梯度洗脱,流速为0.2 mL·min^(-1),柱温为30℃,检测波长为296 nm。结果:12批生品和12批炮制品的总含量分别为3.18~4.69 mg·g^(-1)和0.91~2.77 mg·g^(-1),指纹图谱中均有9个共有峰,相似度分别为0.963~0.991和0.692~0.988,主成分分析和聚类分析能很好地识别干蟾皮的生品和炮制品。结论:该方法稳定、可靠,可为干蟾皮的质量控制、炮制工艺,进一步开发研究提供科学依据。Objective:To control the quality of Bufonis Corium with chemical pattern,UPLC fingerprints of raw materials and processed products of Bufonis Corium were establish and the contents of the index components of Bufonis Corium,such as gamafotalin,arenobufagin,bufotalidin,bufotaline,cinobufotalin,bufalin,cinobufagin,resibufogenin and 3-arenobufagyl-subericacid were determined.Methods:The methanol extract of dry ecdysis was analyzed by Waters Acquity UPLC BEH chromatographic column(100 mm×2.1 mm,1.7μm)with a mobile phase of acetonitrile-0.5%acetic acid aqueous solution in gradient elution.The flow rate was 0.2 mL·min^(-1),the column temperature was 30℃and the detection was set at 296 nm.Results:The total contents of 12 batches of raw products and 12 batches of processed products were 3.18-4.69 mg·g^(-1)and 0.91-2.77 mg·g^(-1),there were 9 common peaks in the fingerprints,and the similarity were 0.963-0.991 and 0.692-0.988,respectively.The principal component analysis and cluster analysis could identify the raw and processed products of Bufonis Corium.Conclusion:The method is stable and reliable,and can provide scientific basis for quality control,processing technology and further development of Bufonis Corium.

关 键 词：日蟾毒它灵 沙蟾毒精 蟾蜍它里定 蟾毒它灵 华蟾毒它灵 蟾毒灵 华蟾酥毒基 脂蟾毒配基 沙蟾毒精-3-辛二醇辛酯 干蟾皮 UPLC 指纹图谱 化学模式识别 

分 类 号：R917[医药卫生—药物分析学]