DNA银纳米簇在功能核酸荧光生物传感器中的应用  

Application of DNA Silver Nanoclusters in the Fluorescence Biosensors based on Functional Nucleic Acids

作　　者：王君旸 刘争 张茜 孙春燕 李红霞 WANG Junyang;LIU Zheng;ZHANG Qian;SUN Chunyan;LI Hongxia(College of Food Science and Engineering,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学食品科学与工程学院,长春130062

出　　处：《高等学校化学学报》2022年第6期1-15,共15页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20200201218JC);吉林省教育厅“十三五”科学技术研究规划项目(批准号:JJKH20201010KJ);吉林大学杰出青年教师培育项目(批准号:2132020KJC020)资助.

摘　　要：DNA银纳米簇(DNA-AgNCs)是以DNA为模板,通过碱基杂环上的N原子与Ag^(+)结合,用NaBH_(4)将Ag^(+)还原得到的具有荧光性质的新兴纳米探针.由于DNA-AgNCs具有合成方法简单、生物相容性好和荧光发射波长可调等优点,使其在分析检测等领域具有广泛的应用.本文对DNA-AgNCs的合成和荧光性质两个方面进行了综述,分类总结了以DNA-AgNCs为无标记荧光探针在功能核酸荧光生物传感器方面的应用,对其不足与应用潜力进行展望,以期为未来的研究与应用提供借鉴.DNA silver nanoclusters(DNA-AgNCs)are a kind of fluorescent nanoprobes obtained by reducing Ag^(+) with NaBH_(4) using DNA as template,because the N atoms on the base heterocyclic are combined with Ag^(+).Due to the advantages of simple synthesis method,good biocompatibility and adjustable fluorescence emission wavelength,DNA-AgNCs have been widely used in the fields of analysis and detection.In this paper,the application of DNA-AgNCs as label-free fluorescent probes in functional nucleic acids biosensing detection is classified and summarized.In addition,conclusive views on the deficiencies and application potential are put forward,which provide references for future research and application.

关 键 词：DNA银纳米簇 功能核酸 荧光 生物传感器 

分 类 号：O657.3[理学—分析化学]