The sampling efficiencies of commercial nasopharyngeal swabs  

作　　者：Ying Wang Geng Wang Lulu Zhang Lili Ren Jianwei Wang 

机构地区：[1]NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory,IPB,CAMS-Fondation Mérieux,Institute of Pathogen Biology(IPB),Chinese Academy of Medical Sciences(CAMS)&Peking Union Medical College,Beijing 100730,China [2]Key Laboratory of Respiratory Disease Pathogenomics,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China

出　　处：《Biosafety and Health》2022年第2期66-69,共4页生物安全与健康（英文）

基　　金：funded in part by the Chinese Academy of Medical Sciences(CAMS);Innovation Fund for Medical Sciences(2020-I2M-2-013);Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019PT310029);funding from Beijing Municipal Science and Technology Commission(Z201100008920008).

摘　　要：Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019(COVID-19)diagnosis.The samples collected from the nasopharynx are preferred for viral nucleic acids detection.Commercial nasopharyngeal swabs(NPSs)are the major factor that influences the sampling quality.We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene(GAPDH)retrieved from the swabs using the RT-PCR method.Significant different concentrations of GAPDH were detected,ranged from 4.36×10^(8) copies/mL to 6.98×10^(10) copies/mL among the five swabs(P<0.05).The designation of the swab head,with or without tip expansion,had limited influence on the collection efficiency.The discrepancy among the NPSs emphasized the improvement of the swab head material.

关 键 词：Nasopharyngeal swabs Nucleic acids EFFICIENCY DESIGNATION 

分 类 号：R440[医药卫生—诊断学] R563.1[医药卫生—临床医学]