成簇的规律间隔的短回文重复序列/成簇的规律间隔的短回文重复序列关联系统在核酸检测中的应用进展  

Application Progress of Clustered Regularly Interspaced Short Palindromic Repeats/Clustered Regularly Interspaced Short Palindromic Repeats-Associated System in Nucleic Acid Assay

作　　者：李宗祥 王金林 王佳芮 罗继全 Li Zongxiang;Wang Jinlin;Wang Jiarui;Luo Jiquan(Sinocare Inc.,Changsha Hunan 410000,China)

机构地区：[1]三诺生物传感股份有限公司,湖南长沙410000

出　　处：《医疗装备》2022年第13期187-192,共6页Medical Equipment

基　　金：湖南省科技创新决策咨询暨软科学重点项目(2019ZL2030)。

摘　　要：成簇的规律间隔的短回文重复序列(CRISPR)/CRISPR关联基因(Cas)系统是目前发现存在于大多数细菌与所有的古生菌中的一种后天免疫系统,以消灭外来的质体或者噬菌体,其中CRISPR/Cas9、CRISPR/Cas12和CRISPR/Cas13系统在核酸检测领域得到应用。该研究简要介绍了CRISPR/Cas系统的分类、作用机制及在核酸检测中的应用。Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated proteins(Cas)system is a kind of acquired immune system found in most bacteria and all archaea so far to defense against foreign plastid or phage.Among them,CRISPR/Cas9,CRISPR/Cas12 and CRISPR/Cas13 are used in nucleic acid assay field.This study briefly introduced the classification,mechanism and application in nucleic acid assay of CRISPR/Cas system.

关 键 词：成簇的规律间隔的短回文重复序列/成簇的规律间隔的短回文重复序列关联系统 核酸检测 应用进展 

分 类 号：G353.11[文化科学—情报学]