An adjusted ELISpot-based immunoassay for evaluation of SARS-CoV-2-specific T-cell responses  

作　　者：Hao Lin Jie Zhang Shaobo Dong Yaning Liu Peipei Liu George F.Gao William J.Liu Guizhen Wu 

机构地区：[1]National Health Commission Key Laboratory of Biosafety,Research Units of Adaptive Evolution and Control of Emerging Viruses,Chinese Academy of Medical Sciences,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention(China CDC),Beijing 102206,China [2]Macheng Center for Disease Control and Prevention,Huanggang 438300,China [3]Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China

出　　处：《Biosafety and Health》2022年第3期179-185,共7页生物安全与健康（英文）

基　　金：supported by the National Natural Science Foundation of China(82161148008 and 81971501);the National Key Research and Development Program of China(2021YFC2301400);Beijing Municipal Science and Technology Project(Z211100002521015&Z211100002521017);In addition,W.J.L.is supported by the Excellent Young Scientist Program of the National Natural Science Foundation of China(81822040).

摘　　要：Like antibody evaluation,using an effective antigen‐specific T‐cell immunity assessment method in coronavirus disease 2019(COVID‐19)patients,survivors and vaccinees is crucial for understanding the immune persistence,prognosis assessment,and vaccine development for COVID‐19.This study evaluated an empirically adjusted enzyme‐linked immunospot assay for detecting severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)‐specific T‐cell immunity in 175 peripheral blood samples from COVID‐19 convalescents and healthy individuals.Results of viral nucleic acid were used as the gold standard of infection confirmation.The SARS‐CoV‐2M peptide pool had higher sensitivity of 85%and specificity of 71%for the single peptide pool.For combined peptide pools,the parallel evaluation(at least one of the peptide pools is positive)of total peptide pools(S1&S2&M&N)had higher sensitivity(up to 93%),and the serial evaluation(all peptide pools are positive)of total peptide pools had higher specificity(up to 100%).The result of the serial evaluation was better than that of the parallel evaluation as a whole.The detection efficiency of M and N peptide pool serial evaluation appeared the highest,with a sensitivity of 80%and specificity of 93%.This T‐cell immunity detection assay introduced in this report can achieve high operability and applicability.Therefore,it can be an effective SARS‐CoV‐2‐specific cellular immune function evaluation method.

关 键 词：SARS‐CoV‐2 COVID‐19 T‐cell response ELISpot assay Sensitivity SPECIFICITY 

分 类 号：R563.1[医药卫生—呼吸系统] R392[医药卫生—内科学]