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作 者:刘益勇 周亚东 申磊 赵恩鹏 杨旭[1] LIU Yi-yong;ZHOU Ya-dong;SHEN Lei(School of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009)
机构地区:[1]扬州大学园艺与植物保护学院,江苏扬州225009
出 处:《安徽农业科学》2022年第13期52-55,58,共5页Journal of Anhui Agricultural Sciences
基 金:国家重点研发计划项目子课题(2019YFD1000305-08)。
摘 要:以低温敏感型材料“荷包茄”嫁接低温耐受型材料“喀西茄”砧木(处理组),“荷包茄”自我嫁接(对照组),进行低温胁迫(1℃)处理,分析其表型变化并分别测定其叶绿素、可溶性糖、丙二醛的含量、电导率变化以及过氧化氢酶(CAT)活性,以此分析接穗低温耐受能力变化。结果表明,1℃处理5 d后对照组比处理组萎蔫明显,冷害指数统计表明处理组冷害指数比对照组低33%。接穗叶片叶绿素a、b含量在处理5 d后比对照组分别高约72%、100%;可溶性糖含量在处理3 d时上升至峰值,处理组比对照组高约65%,之后呈下降趋势;丙二醛含量呈先上升后下降的趋势,在2 d时升至峰值,处理组比对照组低约13.7%;电导率在处理后一直呈上升趋势,在处理5 d时处理组比对照组低约5%;CAT活性并无规律性变化,但处理组一直显著高于对照组。综合分析可知,以低温耐受型品种“喀西茄”为砧木进行嫁接可以在一定程度上提高低温敏感型品种“荷包茄”的低温耐受性。The low temperature sensitive material‘Hebao eggplant’was grafted by grafting the low temperature tolerant material‘Kaxi eggplant’rootstock(treatment group)and‘poached eggplant’was self-grafted(control group),proceed low temperature stress(1℃),the phenotypic changes were analyzed,and the contents of chlorophyll,soluble sugar,malondialdehyde,electrical conductivity and catalase(CAT)activity were determined,and the change of low temperature tolerance of scion was analyzed.The results showed that after 5 days of treatment at 1℃,the control group had more obvious wilting than the treatment group,and the chilling injury index statistics showed that the chilling injury index of the treatment group was 33%lower than the control group.After 5 days of treatment,the content of chlorophyll a and b in scion leaves was about 72%and 100%higher than that in the control group,respectively;the content of soluble sugar in the treatment group increased to the peak after 3 days,and the content of soluble sugar in the treatment group was about 65%higher than that in the control group,and then decreased;the content of MDA increased at first and then decreased,reaching the peak on the 2nd day,and the content of soluble sugar in the treatment group was about 13.7%lower than that in the control group.The electrical conductivity showed an upward trend after treatment,and the CAT activity of the treatment group was about 5%lower than that of the control group after 5 days of treatment,and there was no regular change,but the treatment group was significantly higher than the control group.The comprehensive analysis showed that the low temperature tolerance of low temperature sensitive variety‘Hebao eggplant’could be improved to some extent by grafting with low temperature tolerant variety‘Kaxi eggplant’as rootstock.
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