小鼠室管膜细胞体外培养及多纤毛形成过程分析  

Primary culture of mouse ependymal cells and analysis of the multicilia formation process

作　　者：张源 武慧渊 ZHANG Yuan;WU Huiyuan(School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学药物科学与技术学院,天津300072

出　　处：《中国实验动物学报》2022年第3期370-375,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：天津市自然科学基金(17JCYBJC41900);天津大学自主创新基金(2021XT-0003)。

摘　　要：目的利用体外原代培养小鼠室管膜细胞(MEPC),表征其分化过程形态特征,纤毛的形成及摆动方式。方法通过将分离的新生小鼠端脑组织细胞进行特殊培养和分化,获得具有生理功能的MEPC。利用免疫组化方法跟踪MEPC分化过程中纤毛的形成,并在活细胞状态观察其纤毛摆动模式。结果分离的端脑细胞在特殊的培养条件下快速增殖,经过诱导分化可以得到具有多纤毛结构的MEPC。结论本研究利用改进的原代MEPC培养方案,成功获得具有免疫组化特征及正常多纤毛摆动特性的MEPC,并揭示了分化各个时期纤毛及相关结构的形态特征,为以MEPC为模型的研究奠定了基础。Objective To use a modified protocol to obtain in vitro-cultured mouse ependymal cells(MEPCs)and to assess the morphological change of MEPCs during differentiation,the process of cilia formation,and the ciliary motility of mature MEPCs.Methods MEPCs with physiological functions were obtained by specialized culture and induced differentiation from isolated neonatal mouse telencephalon cells.Cilia formation and maturation of MEPCs were confirmed by immunocytochemistry.The ciliary beating pattern was recorded using a microscope equipped with a high-speed camera.Results The isolated telencephalon cells proliferated rapidly under culture conditions,and MEPCs with functional multicilia were obtained through induced differentiation.Conclusions Using a modified in vitro culture protocol,MEPCs with proper immunohistochemical characteristics and normal multicilia motility were successfully obtained.The process of multicilia formation at various stages of MEPC differentiation was revealed,providing a benchmark for further study using MEPCs as a model.

关 键 词：室管膜细胞 动纤毛 小鼠 原代培养 

分 类 号：Q95-33[生物学—动物学]