circRNA_000203在HIV-1感染儿童疾病进展的表达与临床意义  

Expression and clinical significance of circRNA_000203 in disease progression of HIV-1-infected children

作　　者：韦武均 梁楚晗 黄春传[1] 黄美金[2] 韦安吉 张英杰 黄晶晶 黎作茶[1] 王春芳[1] WEI Wujun;LIANG Chuhan;HUANG Chunchuan;HUANG Meijin;WEI Anji;ZHANG Yingjie;HUANG Jingjing;LI Zuocha;WANG Chunfang(Department of Laboratory Medicine,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Infectious Diseases,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Health Care,Maternal and Child Health Hospital of Baise,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院附属医院医学检验科,广西百色533000 [2]右江民族医学院附属医院感染性疾病科,广西百色533000 [3]广西百色市妇幼保健院保健科,广西百色533000

出　　处：《右江医学》2022年第6期424-428,共5页Chinese Youjiang Medical Journal

基　　金：国家自然科学基金(81960303);百色市科学研究与技术开发计划项目(百科20194701,百科20213301,百科20213242);广西卫生健康委员会自筹经费科研课题(Z20190202);右江民族医学院校级科研课题(yy2019ky008);广西高校中青年教师科研基础能力提升项目(2021KY0538)。

摘　　要：目的检测环状RNA(circRNA)_000203在人类免疫缺陷病毒1型(human immunodeficiency virus type 1,HIV-1)感染儿童外周血单个核细胞中表达,探讨其表达与HIV-1感染儿童的临床意义。方法收集71例HIV-1感染儿童与71例体检正常儿童外周血,分离单个核细胞,采用RT-PCR检测单个核细胞中circRNA_000203表达,统计分析HIV-1感染儿童与正常组表达差异,以及HIV-1不同进展期的表达差异,并分析circRNA_000203表达与CD4细胞数、病毒载量的相关性。结果HIV-1感染的儿童组中circRNA_000203的相对表达量显著高于对照组(P<0.001),HIV进展患儿组中circRNA_000203的相对表达量高于HIV长期未进展患儿组(P<0.001);而ART(antiretroviral therapy)无响应患儿组中circRNA_000203的相对表达量高于ART响应患儿组(P<0.001)。HIV-1感染儿童中circRNA_000203相对表达量与CD4细胞数成反比(R=-0.332,P=0.005),与HIV-1病毒载量成正比(R=0.390,P<0.001)。结论circRNA_000203与HIV-1感染相关,且可能与HIV-1感染预后不良相关,可能是HIV-1感染儿童的潜在诊断指标。Objective To detect the expression of circular RNA(circRNA)_000203 in peripheral blood mononuclear cells of children infected with human immunodeficiency virus type 1(HIV-1),so as to explore the clinical significance of its expression on HIV-1 infected children.Methods The peripheral blood of 71 HIV-1 infected children and 71 normal children with physical examination were collected.Mononuclear cells were isolated,circRNA_000203 expression in mononuclear cells were detected by RT-PCR,the difference of expressions between HIV-1 infected children and normal group as well as the difference of expressions of HIV-1 at different progressive stages were statistically analyzed.In addition,correlation between the expression of circRNA_000203,the number of CD4 cells and viral load was analyzed.Results The relative expression of circRNA_000203 in HIV-1 infected children group was significantly higher than that in control group(P<0.001),and the relative expression of circRNA_000203 in children with HIV progression was significantly higher than that in children with long-term non progress of HIV(P<0.001).However,the relative expression of circRNA_000203 was higher in ART(antiretroviral therapy)unresponsive group than in ART responsive group(P<0.001).The relative expression of circRNA_000203 in HIV-1 infected children was inversely proportional to the number of CD4 cells(R=-0.332,P=0.005),and directly proportional to the viral load of HIV-1(R=0.390,P<0.001).Conclusion CircRNA_000203 is associated with HIV-1 infection and may be associated with poor prognosis of HIV-1 infection,which may be a potential diagnostic indicator of HIV-1 infected children.

关 键 词：人类免疫缺陷病毒 环状RNA 儿童 诊断指标 

分 类 号：R512.91[医药卫生—内科学]