黄毛草莓叶片愈伤组织诱导研究  

Study on Callus Induction of Fragaria nilgerrensis Leaves

作　　者：钱邵函 刘倩文 武春霞 QIAN Shaohan;LIU Qianwen;WU Chunxia(College of Horticulture Landscape,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农业科学》2022年第6期39-42,共4页Tianjin Agricultural Sciences

基　　金：天津市级大学生创新创业项目(201910061058)。

摘　　要：为建立黄毛草莓叶片繁殖体系,筛选诱导愈伤组织的最适培养基,以黄毛草莓幼嫩叶片为外植体,研究了消毒时间、激素组合、光暗条件对黄毛草莓叶片愈伤组织诱导的影响。结果表明:黄毛草莓叶片最佳的外植体消毒处理为0.1%的升汞消毒6~8 min;黄毛草莓叶片愈伤组织褐化严重,经15%抗坏血酸(Vc)溶液浸泡20 min可显著降低褐化率,提高出愈率;愈伤组织诱导的最适培养基组合为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA和MS+1.0 mg·L^(-1) TDZ+0.1 mg·L^(-1) IBA;暗培养10 d出愈率最高,可达到87.5%。因此,黄毛草莓叶片愈伤组织诱导最佳培养方法为:MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1)蔗糖+7 g·L^(-1)琼脂,培养基pH值为5.6,15%Vc溶液浸泡20 min,出愈率可达到95.0%。In order to establish an efficient method for plant regeneration from leaf explant of Fragaria nilgerrensis plantlet the most suitable medium for inducing callus was screened.The leaves of Fragaria nilgerrensis plantlet were used for studying the effects of disinfection time,plant growth regulator concentration and ratio,different incubation time in dark,on callus induction in leaves were studied using the young and tender leaves of Fragaria nilgerrensis as explants.The results showed that the optimal explant disinfection treatment for 0.1%of the leaves of Fragaria nilgerrensis was 0.1%mercury disinfection for 6-8 minutes,and the callus of Fragaria nilgerrensis leaf was severely browned,and the browning rate and callus induction rate could be significantly reduced by soaking in 15%ascorbic acid(Vc)solution for 20 minutes.The optimal medium combination for callus induction was MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA and MS+1.0 mg·L^(-1) TDZ+0.1 mg·L^(-1) IBA for 10 d in dark,the callus induction frequency was up to 87.5%.The best cultivation method is MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1) sucrose+7 g·L^(-1) Agar-Agar,medium pH 5.6 and soaks in 15%Vc solution for 20 minutes,the callus induction frequency is up to 95%.

关 键 词：黄毛草莓 叶片 愈伤组织 诱导 

分 类 号：S668.4[农业科学—果树学]