临床极重症钩虫感染1例  

A case of severe hookworm infection

作　　者：马玲辉 Dibo Nouhoum 王婉妮 张志珊[1] 侯循亚 喻鑫玲 邓维成 管洁 黄帅钦 吴翔 MALinghui;Dibo Nouhoum;WANGWanni;ZHANG Zhishan;HOU Xunya;YU Xinling;DENGWeicheng;GUAN Jie;HUANG Shuaiqin;WU Xiang(Department of Laboratorial Examination,Quanzhou First Affiliated Hospital of Fujian Medical University,Quanzhou Fujian 362000;Department of Prevention and Control,Hunan Institute of Schistosomiasis Control,Yueyang Hunan 414021;Key Laboratory of Immunization and Transmission Control of Schistosomiasis in Hunan Province,Yueyang Hunan 414125;Department of Pathology,Hunan Aerospace Hospital,Changsha 410006;Departmentof Parasitology,School of Basic Medical Sciences,Central South University,Changsha 410078,China)

机构地区：[1]福建医科大学附属泉州第一医院检验科,福建泉州362000 [2]湖南省血吸虫病防治研究所检测防治部,湖南岳阳414021 [3]血吸虫病免疫与传播控制湖南省重点实验室,湖南岳阳414125 [4]湖南省航天医院病理科,长沙410006 [5]中南大学基础医学院寄生虫学系,长沙410078

出　　处：《中南大学学报（医学版）》2022年第6期814-822,共9页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(82072306)。

摘　　要：随着我国卫生条件的改善,钩虫的感染率得到了大幅度的降低,重症感染的患者已极其罕见。福建医科大学附属泉州第一医院检验科通过形态学检查和分子生物学方法,确诊了1例极重症钩虫感染的患者。该患者粪便直接涂片法和钩蚴培养法可见大量虫卵,从其虫卵和幼虫形态学上判断为钩虫,改良加藤法计数虫卵为每克粪便60840个,属于极重度感染状态;同时还提取了粪便中虫卵的DNA,采用半巢式RT-PCR对其进行虫种鉴定,结果显示为美洲钩虫。With the improvement of sanitation,the infection rate of hookworm is greatly reduced and the severe infected case is rarely reported.Combined morphological and molecular biological examinations,a severe hookworm infection patient was diagnosed in Department of Laboratorial Examination,Quanzhou First Affiliated Hospital of Fujian Medical University.The morphological methods such as direct fecal smear microscopy,saturated brine flotation and hookworm larvae culture methods were used to identify the eggs and larvae from stool samples of the patient.There were a large number of hookworm eggs in patient’s stool samples,and the average count was 60840 per gram by modified Kato method,which belonged to severe hookworm infection.Meanwhile,to distinguish the hookworm species,the semi-nested RT-PCR assay was employed to detect hookworm internal transcribed spacer series from eggs in patient’s stool samples,and the result showed that the hookworm species was confirmed to be Necator americanus.

关 键 词：钩虫 虫种鉴定 极重症感染 半巢式RT-PCR 

分 类 号：R532.12[医药卫生—内科学]