冬虫夏草提取液对^(60)Coγ射线辐照小鼠睾丸组织氧化损伤的保护作用  

Effects of Cordyceps sinensis exact on the apoptosis in testicular tissue induced by^(60)Coγirradiation

作　　者：魏闪闪 王婷[2] 彭伟彪 汪家春 冯旭 苏笠 储智勇 张阵阵 Wei Shanshan;Wang Ting;Peng Weibiao;Wang Jiachun;Feng Xu;Su Li;Chu Zhiyong;Zhang Zhenzhen(Naval Medical Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军特色医学中心,上海200433 [2]江西中医药大学药学院 [3]上海大学转化医学研究院

出　　处：《海军医学杂志》2022年第4期364-369,共6页Journal of Navy Medicine

基　　金：上海市2021年度“科技创新行动计划”生物医药科技支撑专项项目(21S21902800);上海市科学技术委员会技术标准专项项目(16DZ0501400)。

摘　　要：目的研究冬虫夏草提取液(CSE)对辐照诱导小鼠睾丸组织氧化损伤的保护机制。方法50只C57BL/6雄性小鼠按数字表法随机分为5组,分别为未辐照组(Control组)、辐照组(Model组)、低剂量CSE组(CSE‐L组)、中剂量CSE组(CSE‐M组)和高剂量CSE组(CSE‐H组),每组各10只。除Control组外,其余组均以单次8 Gy ^(60)Coγ射线全身辐照小鼠建立睾丸损伤模型。CSE‐L、CSE‐M、CSE‐H组小鼠每日灌胃给药1次,生药剂量分别为312.5、625.0、1250.0 mg/ml,持续给药28 d。检测不同生药剂量的CSE对辐照后小鼠体重、睾丸组织中丙二醛(MDA)水平及超氧化物歧化酶(SOD)活力的影响,并对Control、Model组、CSE‐H组小鼠的睾丸组织进行了苏木精伊红(HE)染色病理学、髓过氧化物酶(MPO)染色、凋亡相关基因的检测。结果与Control组相比,Model组小鼠睾丸组织SOD活力减弱、MDA水平上升,表明造模成功;与Model组相比,不同剂量的CSE均能显著改善上述辐照损伤指标(P<0.05)。HE、MPO染色结果显示,CSE‐H组中辐照诱导的小鼠睾丸组织切片结构损伤明显改善,炎性浸润显著降低。此外,与Control组对比,Model组小鼠睾丸组织抗氧化基因PARP1基因mRNA相对表达量明显上升,GSH‐Px、CAT基因mRNA表达水平明显降低(均P<0.01)。CSE治疗后,PARP1基因mRNA相对表达量明显下降(P<0.01),GSH‐Px基因mRNA相对表达量明显上升(P<0.01)。结论高剂量CSE对电离辐照导致的睾丸组织氧化损伤有保护作用。Objective To study the protective mechanism of Cordyceps sinensis exact(CSE)on the oxidative damage of testicular tissue indduced by ^(60)Coγirradiation in mice.Methods 50 C57BL/6 male mice were randomly divided into 5 groups,i.e.the non‐irradiated group(or the control group),the irradiation group(or the model group),the CSE with low dose group(or the CSE‐L group);the CSE with middle dose group(or the CSE‐M group)and the CSE with high dose group(or the CSE‐H group),each consisting of 10 animals.With exception of the control group,the remaining groups rceived a s single dose of 8 Gy ^(60)Coγtotal‐body irradiation to establish oxidative damage in the testicular tissue.The mice in the CSE‐L,CSE‐M,and CSE‐H groups were gavaged with crude drug doses of 312.5,625.0,1250.0 mg/ml once a day,for a succession of 28 days.Detections were made on the effects of different doses of CSE after irradiation on body mass,malondialdehyde(MDA)and superoxide dismutase(SOD)activity levels of testicular tis‐sues in mice.Detections were also made on the hematoxylin eosin(HE)histopathology,myeloperoxidase(MPO)positive cells and the expression of antioxidant‐related genes in the testicular tissues of the control,model and CSE‐H groups by HE and steining.Results Compared with those of the control group,the level of SOD activity decreased significantly the model group,and the MDA level of testicular tissue elevated in the model group,indicating that success of model establishment.As compared with the model group,different doses of CSE could markedly improve the above damages induced by irradiation(P<0.05).HE and MPO staining shown that the irradiation‐induced structure damage of testicular tissues in the CSE‐H group markedly improved and Inflammatory infiltration significantly decreased.In addition,antioxidant‐related mRNA expressions of PARP1 in the testicular tissues of the mice dramatically increased,GSH‐PX and CAT dramatically reduced(P<0.01),and following CSE treatement,mRNA expressions of PARP1 dramatical

关 键 词：电离辐照 冬虫夏草提取液 睾丸组织 氧化损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]