经鼻给脂多糖对小鼠嗅觉和嗅球及内侧前额叶皮质轻链铁蛋白表达的影响  

EFFECT OF INTRANASAL ADMINISTRATION OF LIPOPOLYSACCHARIDE ON THE OLFACTORY SENSATION AND THE EXPRESSION OF L-FERRITIN IN THE OLFACTORY BULB AND MEDIAL PREFRONTAL CORTEX OF MICE

作　　者：袁玉 马希珍 宋宁[1] 谢俊霞[1] YUAN Yu;MA Xizhen;SONG Ning;XIE Junxia(Institute of Brain Science and Diseases,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学脑科学与疾病研究院,山东青岛266071

出　　处：《青岛大学学报（医学版）》2022年第3期317-320,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32170984)。

摘　　要：目的探讨鼻内滴注脂多糖(LPS)对小鼠嗅觉和嗅球及内侧前额叶皮质中轻链铁蛋白表达的影响。方法8周龄雄性C57BL/6小鼠16只,随机分为对照组和LPS组,每组8只。LPS组双侧鼻孔交替滴注1 g/L的LPS(每只10μL),对照组给予等体积的生理盐水,隔天1次,给药时长为3周。3周后测试两组小鼠的嗅觉功能,采用免疫印迹法检测嗅球中酪氨酸羟化酶(TH)的表达,并检测嗅球及内侧前额叶皮质中轻链铁蛋白的表达。结果与对照组相比,LPS组小鼠没有明显的嗅觉障碍;嗅球中TH表达无明显变化;嗅球中轻链铁蛋白表达升高87%,差异有统计学意义(t=4.486,P<0.05);内侧前额叶皮质中轻链铁蛋白水平升高84%,差异有统计学意义(t=2.391,P<0.05)。结论经鼻给LPS能够引起嗅球及内侧前额叶皮质中铁含量上升,但没有造成明显的嗅觉障碍及嗅球内多巴胺能神经元损伤。Objective To investigate the effect of intranasal instillation of lipopolysaccharide(LPS)on the olfactory sensation and the expression of L-ferritin in the olfactory bulb(OB)and medial prefrontal cortex(mPFC)of mice.Methods Sixteen male C57BL/6 mice aged 8 weeks were randomly divided into control group and LPS group,with 8 mice in each group.The LPS group was instilled with 1 g/L LPS alternately to bilateral nostrils at 10μL/animal,once every other day for 3 weeks,and the control group was instilled with the same volume of normal saline.Three weeks later,the olfactory function of mice in the two groups was tested,and the expression level of tyrosine hydroxylase(TH)in the OB and the expression level of L-ferritin in the OB and mPFC were detected by Western blot.Results Compared with the control group,the LPS group had no obvious olfactory disorder,no significant change in the expression level of TH in the OB,an increase of 87%in the expression level of L-ferritin in the OB(t=4.486,P<0.05),and an increase of 84%in the expression level of L-ferritin in the mPFC(t=2.391,P<0.05).Conclusion Intranasal administration of LPS can increase the iron content in the OB and mPFC,but does not cause obvious olfactory disorder and dopaminergic neuron damage in the OB.

关 键 词：脱辅铁蛋白质类 脂多糖类 多巴胺能神经元 嗅球 额叶前皮质 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]