Ghsr^(-/-)小鼠黑质ATP敏感性钾通道亚基表达改变  

CHANGE IN THE EXPRESSION OF ATP-SENSITIVE POTASSIUM CHANNEL SUBUNITS IN THE SUBSTANTIA NIGRA OF Ghsr^(-/-) MICE

作　　者：吕吉荣 肖雪 焦倩[1] 陈曦[1] 杜希恂[1] 姜宏[1] LÜ Jirong;XIAO Xue;JIAO Qian;CHEN Xi;DU Xixun;JIANG Hong(State Key Disciplines:Physiology(in Incubation),Department of Physiology,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东青岛266071

出　　处：《青岛大学学报（医学版）》2022年第3期329-332,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31771110);山东省自然科学基金重大基础研究项目(ZR2019ZD31);山东省自然科学基金青年基金(ZR2020QH125)。

摘　　要：目的探讨生长激素促分泌素受体1a(GHSR1a)基因敲除小鼠黑质ATP敏感性钾通道(K_(ATP)通道)亚基的表达变化。方法选取5只3月龄GHSR1a敲除(Ghsr^(-/-))小鼠和5只同窝野生型(WT)小鼠,应用蛋白免疫印迹和实时荧光定量PCR方法检测小鼠黑质K_(ATP)通道组成亚基SUR1、SUR2B、Kir6.2的蛋白和mRNA水平。结果与WT组相比,Ghsr^(-/-)组小鼠黑质K_(ATP)通道组成亚基SUR1蛋白表达水平降低(t=3.389,P<0.05),而SUR2B、Kir6.2蛋白表达无明显变化(P>0.05)。两组小鼠黑质K_(ATP)通道组成亚基SUR1、SUR2B、Kir6.2的mRNA表达水平差异均无显著性(P>0.05)。结论GHSR1a基因敲除会导致黑质K_(ATP)通道组成亚基SUR1蛋白的表达降低。Objective To investigate the change in the expression of ATP-sensitive potassium channel(K_(ATP) channel)subunits in the substantia nigra of mice with growth hormone secretagogue receptor 1a(GHSR1a)gene knockout.Methods Five GHSR1a-knockout(Ghsr^(-/-))mice and five wild-type(WT)littermates were selected,and Western blotting and quantitative real-time PCR were used to measure the protein and mRNA expression levels of the SUR1,SUR2B,and Kir6.2 subunits of K_(ATP) channel in the substantia nigra.Results Compared with the WT group,the Ghsr^(-/-) group had a significant reduction in the protein expression level of the SUR1 subunit of K_(ATP) channel in the substantia nigra(t=3.389,P<0.05),while there were no significant changes in the protein expression levels of SUR2B and Kir6.2 subunits(P>0.05).There were no significant differences between the two groups in the mRNA expression levels of SUR1,SUR2B,and Kir6.2 of K_(ATP) channel(P>0.05).Conclusion GHSR1a gene knockout can reduce the protein expression of the SUR1 subunit of K_(ATP) channel in the substantia nigra.

关 键 词：受体 胃促生长素 黑质 K_(ATP)通道 小鼠 基因敲除 

分 类 号：R338.2[医药卫生—人体生理学]