帕金森病模型小鼠PV和NOS阳性神经元数目变化  

CHANGES IN THE NUMBER OF NEURONS WITH POSITIVE PARVALBUMIN AND NITRIC OXIDE SYNTHASE IN THE ZONA INCERTA OF MICE WITH PARKINSON’S DISEASE

作　　者：曹中凯 陈凤华 谢俊霞 石丽敏 CAO Zhongkai;CHEN Fenghua;XIE Junxia;SHI Limin(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2022年第3期345-348,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32171132)。

摘　　要：目的探讨1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型小鼠未定带小清蛋白(PV)和一氧化氮合酶(NOS)阳性神经元的数目变化。方法7周龄雄性C57BL/6小鼠12只,随机分为对照组及MPTP组,每组6只。MPTP组采用连续5 d腹腔注射MPTP(30 mg·kg^(-1)·d^(-1))的方法制备PD小鼠模型,对照组以等量生理盐水代替MPTP。采用免疫荧光技术观察黑质区酪氨酸羟化酶(TH)阳性神经元及未定带PV、NOS阳性神经元的表达变化。结果与对照组相比,MPTP组小鼠黑质区TH阳性神经元的数目明显减少,差异有统计学意义(t=6.888,P<0.01);未定带PV阳性神经元和NOS阳性神经元数目均明显减少,差异有显著性(t=3.618、15.390,P<0.01)。结论MPTP诱导PD模型小鼠未定带PV和NOS阳性神经元数目明显下降。Objective To investigate the changes in the number of neurons with positive parvalbumin(PV)and nitric oxide synthase(NOS)in the zona incerta of mice with Parkinson’s disease(PD)induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods A total of 12 male C57BL/6 mice,aged 7 weeks,were randomly divided into control group and MPTP group,with 6 mice in each group.The mice in the MPTP group were given intraperitoneal injection of MPTP(30 mg·kg^(-1)·d^(-1))for 5 consecutive days to establish a mouse model of PD,and those in the control group were given an equal volume of normal saline.Immunofluorescence assay was used to observe the changes in the expression of tyrosine hydroxylase(TH)-positive neurons in the substantia nigra and PV-and NOS-positive neurons in the zona incerta.Results Compared with the control group,the MPTP group had significant reductions in the number of TH-positive neurons in the substantia nigra(t=6.888,P<0.01)and the number of PV-and NOS-positive neurons in the zona incerta(t=3.618,15.390;P<0.01).Conclusion MPTP can induce a significant reduction in the number of PV-and NOS-positive neurons in the zona incerta of mice with PD.

关 键 词：帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 未定带 小白蛋白 一氧化氮合酶 神经元 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]