枸橼酸铁铵对少突胶质细胞铁代谢相关蛋白表达影响  

EFFECT OF FERRIC AMMONIUM CITRATE ON THE EXPRESSION OF IRON METABOLISM-RELATED PROTEINS IN OLIGODENDROCYTES

作　　者：王冰菁 张惠琴[2] 谢俊霞[3] 王俊 WANG Bingjing;ZHANG Huiqin;XIE Junxia;WANG Jun(Department of Physiology,School of Basic Medicine,Medical College of Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学教研室,山东青岛266071 [2]中国石油大学(华东)校医院 [3]青岛大学脑科学与疾病研究院

出　　处：《青岛大学学报（医学版）》2022年第3期367-369,共3页Journal of Qingdao University(Medical Sciences)

基　　金：山东省重点研发课题(2019GSF108224)。

摘　　要：目的探讨枸橼酸铁铵(FAC)对未分化MO3.13少突胶质细胞铁代谢相关蛋白表达的影响。方法将MO3.13细胞分为对照组和FAC组。对照组给予细胞培养液处理,FAC组用加入100μmol/L FAC的细胞培养液处理。处理24 h后采用蛋白质免疫印迹实验检测两组细胞转铁蛋白受体1(TfR1)和铁转运蛋白1(FPN1)的表达水平。结果与对照组相比,FAC组细胞TfR1表达水平降低(t=2.695,P<0.05),FPN1表达水平无明显变化(t=0.210,P>0.05)。结论FAC可引起少突胶质细胞TfR1表达降低,而FPN1表达不变。Objective To investigate the effect of ferric ammonium citrate(FAC)on the expression of iron metabolism-related proteins in undifferentiated MO3.13 oligodendrocytes.Methods MO3.13 cells were divided into control group and FAC group.The cells in the control group were treated with a cell culture medium,and those in the FAC group were treated with the cell culture medium containing 100μmol/L FAC.After treatment for 24 hours,Western blotting was used to measure the expression levels of transferrin receptor 1(TfR1)and ferroportin 1(FPN1)in both groups.Results Compared with the control group,the FAC group had a significant reduction in the expression level of TfR1(t=2.695,P<0.05)and had no significant change in the expression of FPN1(t=0.210,P>0.05).Conclusion FAC can reduce the expression of TfR1 in oligodendrocytes,while the expression of FPN1 remains unchanged.

关 键 词：铁 少突神经胶质 受体 转铁蛋白 转铁蛋白类 

分 类 号：R338.2[医药卫生—人体生理学]