单纯性肥胖者DC负载结核Th优势表位肽后的免疫功能改变探究  

Changes of immune function in patients with simple obesity after DC loaded with Th dominant epitope peptide of tuberculosis

作　　者：黄新亮 伍云云 董博翰 Huang Xinliang;Wu Yunyun;Dong Bohan(School of Basic Medicine,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院基础医学院,安徽芜湖241002

出　　处：《右江民族医学院学报》2022年第3期404-407,422,共5页Journal of Youjiang Medical University for Nationalities

基　　金：安徽高校省级自然科学研究重点项目(KJ2019A0415,KJ2021A0829)。

摘　　要：目的探究单纯性肥胖者DC负载结核杆菌Th优势表位肽后的免疫功能改变机制。方法设肥胖组、健康组、空白组各13例、7例、6例。分离外周静脉血得单核细胞,以细胞因子IL-4和GM-CSF刺激获得DC,对肥胖组和健康组以DC负载结核杆菌的Th优势表位肽制备疫苗,对空白组以DC对照,培养8 d后流式仪鉴定DC的成熟度(表达CD11c、CD83、CD86的DC百分率)。各组DC和自身淋巴细胞按照1∶20比例混合4 d后流式仪鉴定表达活化标志分子ICOS的淋巴细胞、效应CD4+T及CD8+T。并以酶联免疫吸附法检测Th产生的细胞因子IFN-γ、LDH法检测CTL的细胞毒效应。结果与健康组及空白组比较,肥胖组表达CD11c、CD83、CD86的DC细胞百分率,活化分子ICOS淋巴细胞、CD4+T、CD8+T百分率及Th产生的细胞因子IFN-γ升高,差异有统计学意义(P<0.05);而肥胖组CTL的细胞毒效应比健康组明显下降,差异有统计学意义(P<0.05)。结论单纯性肥胖者DC负载结核杆菌的Th优势表位肽后,DC成熟度及提呈抗原功能增强。且诱导的活化的自身淋巴细胞及分化的效应CD4+T和CD8+T增多。其诱导Th产生的IFN-γ水平上升而CTL的细胞毒效应下降。Objective To explore the mechanism of immune function changes in patients with simple obesity after their DC is loaded with Th dominant epitope peptide of tuberculosis.Methods There were 13 cases in the obese group,7 cases in the healthy group and 6 cases in the blank group.Monocytes were isolated from their peripheral venous blood and these moncytes were stimulated with cytokine IL-4 and GM-CSF to obtain DC.For the obese group and the healthy group,the vaccine was prepared with the DC loaded with Th dominant epitope peptide of mycobacterium tuberculosis.For the blank group,DC was used as the control,and the maturity of DC(percentages of DC expressing CD11c,CD83 and CD86)was determined by the flow cytometry after 8 days of culture.After 4 days of mixture of DC and autolymphocytes at a ratio of 1∶20 in each group,the lymphocytes,effector CD4+T and CD8+T which expressed the activated molecule ICOS,were identified by the flow cytometry.The enzyme-linked immunosorbent assay was adopted to detect the cytokine IFN-r produced by Th and the cytotoxic effect of CTL was detected by LDH method.Results Compared with the healthy group and the blank group,the obese group had significantly increased percentages of DC cells expressing CD11c,CD83 and CD86,as well as percentages of lymphocytes,CD4+T,CD8+T of the activated ICOS and increased IFN-r produced by Th,showing significant differences(P<0.05).The cytotoxic effect of CTL in the obesity group decreased more obviously than that in the healthy group,and the difference was significant(P<0.05).Conclusion The maturity and antigen presenting function of DC inpatients with only obesityare enhanced after DC is loaded with Th dominant epitope peptide of tuberculosis.And there is an increase in the DC-induced activated autolymphocytes and differentiated effectors CD4+T and CD8+T.DC induces an increase in the level of IFN-γproduced by Th,while an decrease in the cytotoxic effect of CTL.

关 键 词：单纯性肥胖 树突状细胞 表位肽 免疫 

分 类 号：R392[医药卫生—免疫学]