川芎嗪调控NF-κB信号通路对银屑病HaCaT细胞模型趋化因子和炎症因子表达影响  

Effect of ligustrazine regulating NF-κB signaling pathway on expressions of chemokines and inflammatory factors in a HaCaT cell model of psoriasis

作　　者：王生[1] 刘洋[1,2] 张晶[1] WANG Sheng;LIU Yang;ZHANG Jing(Department of Dermatology,Tangshan Workers'Hospital Affiliated to Hebei Medical University,Tangshan 063000,China)

机构地区：[1]河北医科大学附属唐山工人医院皮肤科,唐山063000 [2]开滦总医院,唐山063000

出　　处：《中国免疫学杂志》2022年第8期952-957,共6页Chinese Journal of Immunology

摘　　要：目的:探讨川芎嗪调控NF-κB信号通路对银屑病HaCaT细胞模型趋化因子和炎症因子表达的影响。方法:HaCaT细胞分为Control、Model(10 ng/ml的TNF-α和IFN-γ处理)、TMP-L(0.1 mg/ml的川芎嗪、10 ng/ml的TNF-α和IFN-γ处理)、TMP-M(0.2 mg/ml的川芎嗪、10 ng/ml的TNF-α和IFN-γ处理)、TMP-H(0.4 mg/ml的川芎嗪、10 ng/ml的TNF-α和IFN-γ处理)、TMP-M+PMA组(1μmol/L的NF-κB信号激活剂PMA、0.2 mg/ml的川芎嗪、10 ng/ml的TNF-α和IFN-γ处理)。qRT-PCR检测Eotaxin、RANTES、TARC mRNA表达,ELISA法检测IL-6、IL-4、IL-13含量,Western blot检测p65、IκBα蛋白表达。结果:与Control组相比,Model组HaCaT细胞模型中Eotaxin、RANTES、TARC mRNA表达水平升高(1.00±0.09 vs 3.69±0.25,1.00±0.10 vs4.51±0.28,1.00±0.11 vs 2.96±0.21,P<0.05),细胞分泌的IL-6、IL-4、IL-13增多[(96.32±8.87)pg/ml vs(275.14±26.31)pg/ml,(23.61±1.47)pg/ml vs(108.62±11.96)pg/ml,(5.58±0.42)pg/ml vs(26.35±2.47)pg/ml,P<0.05],p65蛋白表达水平升高(0.23±0.02 vs 0.86±0.08,P<0.05),IκBα蛋白表达水平降低(0.96±0.09 vs 0.25±0.04,P<0.05)。与Model组相比,TMP-L、TMP-M、TMP-H组HaCaT细胞模型中Eotaxin、RANTES、TARC mRNA表达水平依次降低(3.69±0.25 vs 2.75±0.14、2.23±0.16、1.40±0.11,4.51±0.28 vs 3.27±0.36、2.23±0.17、1.80±0.12,2.96±0.21 vs 2.03±0.14、1.62±0.12、1.14±0.11,P<0.05),细胞分泌的IL-6、IL-4、IL-13减少[(275.14±26.31) pg/ml vs (204.15±13.62) pg/ml、(162.01±14.81) pg/ml、(117.62±10.30) pg/ml,(108.62±11.96) pg/ml vs(80.43±7.79)pg/ml、(53.24±4.11)pg/ml、(31.20±2.26)pg/ml,(26.35±2.47)pg/ml vs(14.20±1.22)pg/ml、(10.66±1.04)pg/ml、(6.42±0.50)pg/ml,P<0.05],细胞中p65蛋白表达水平降低(0.86±0.08 vs 0.56±0.05、0.45±0.04、0.30±0.03,P<0.05),IκBα蛋白表达水平升高(0.25±0.04 vs 0.52±0.06、0.63±0.06、0.79±0.07,P<0.05)。与TMP-M组相比,TMP-M+PMA组HaCaT细胞模型中Eotaxin、RANTES、TARC mRNA水平升高(1.00±0.08 vs 1.86±0.12,1.00±0.10 vs 2.64±0.23,1.00±0.09 vObjective:To investigate the effect of ligustrazine-regulated NF-κB signaling pathway on expressions of chemokines and inflammatory factors in a HaCaT cell model of psoriasis.Methods:HaCaT cells were divided into Control,Model(10 ng/ml TNF-αand IFN-γ treatment),TMP-L(0.1 mg/ml ligustrazine,10 ng/ml TNF-α and IFN-γ treatment),TMP-M(0.2 mg/ml ligustrazine,10 ng/ml TNF-α and IFN-γ treatment),TMP-H(0.4 mg/ml ligustrazine,10 ng/ml TNF-α and IFN-γ treatment),TMP-M+PMA group(treated with 1 μmol/L NF-κB signal activator PMA,0.2 mg/ml ligustrazine,10 ng/ml TNF-α and IFN-γ treatment). Expressions of Eotaxin,RANTES,TARC mRNA were detected by qRT-PCR,and IL-6,IL-4,IL-13 contents were detected by ELISA,Western blot detection of p65,IκBα protein expressions.Results:Compared with Control group,expression levels of Eotaxin,RANTES and TARC mRNA in HaCaT cell model of Model group were increased(1.00±0.09 vs 3.69±0.25,1.00±0.10 vs 4.51±0.28,1.00±0.11 vs 2.96±0.21,P<0.05),IL-6,IL-4 and IL-13 secreted by cells increased[(96.32±8.87)pg/ml vs(275.14±26.31)pg/ml,(23.61±1.47)pg/ml vs(108.62±11.96)pg/ml,(5.58±0.42)pg/ml vs(26.35±2.47)pg/ml,P<0.05],p65 protein expression level increased(0.23±0.02vs 0.86±0.08,P<0.05),IκBα protein expression level decreased(0.96±0.09 vs 0.25±0.04,P<0.05). Compared with Model group,expression levels of Eotaxin,RANTES and TARC mRNA in HaCaT cell model of TMP-L,TMP-M and TMP-H groups decreased sequentially(3.69±0.25 vs 2.75±0.14,2.23±0.16,1.40±0.11;4.51±0. 28 vs 3.27±0.36,2.23±0.17,1.80±0.12;2.96±0.21 vs 2.03±0.14,1.62±0.12,1.14±0.11,P<0.05),IL-6,IL-4,IL-13 secreted by cells reduced[(275.14±26.31)pg/ml vs(204.15±13.62)pg/ml,(162.01±14.81)pg/ml,(117.62±10.30)pg/ml;(108.62±11.96)pg/ml vs(80.43±7.79)pg/ml,(53.24±4.11)pg/ml,(31.20±2.26)pg/ml;(26.35±2.47)pg/ml vs(14.20±1.22)pg/ml,(10.66±1.04)pg/ml,(6.42±0.50)pg/ml,P<0.05],expression level of p65 protein in cells decreased(0.86±0.08 vs 0.56±0.05,0.45±0.04,0.30±0.03,P<0.05),and expression level of IκBα pro

关 键 词：川芎嗪 银屑病 HACAT细胞 炎症 核因子-κB信号 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]