NSPs在系统性红斑狼疮患者血清中的表达及临床意义  

Expression and clinical significance of NSPs in serum of patients with systemic lupus erythematosus

作　　者：姜蓉琼 杨桂钊 冯丹[1] 刘静[1] 王丹[1] 陈勇[1,2] 袁国华(指导)[1] JIANG Rongqiong;YANG Guizhao;FENG Dan;LIU Jing;WANG Dan;CHEN Yong;YUAN Guohua(Institute of Rheumatic and Immunology,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院风湿免疫研究所,南充637000 [2]四川省简阳市人民医院风湿科,简阳641400

出　　处：《中国免疫学杂志》2022年第8期992-997,共6页Chinese Journal of Immunology

基　　金：市校科技战略合作专项项目。

摘　　要：目的:探讨血清中3种主要中性粒细胞丝氨酸蛋白酶(NSPs)NE、CG、PR3在系统性红斑狼疮(SLE)患者中的表达及临床意义。方法:ELISA测定66例SLE患者、39例健康对照者血清NE、CG、PR3水平,分析SLE患者NE、CG、PR3水平与病情活动度的相关性,并分析SLE患者NE、CG、PR3水平与实验室指标及临床表现的关系。结果:SLE组血清NE、CG、PR3水平均高于健康对照组(P<0.05)。相关性分析显示,SLE患者血清NE、CG、PR3水平与SLE疾病活动评分呈正相关(r=0.420,0.269,0.475,P<0.05)。血清NE水平升高的SLE患者C4水平更低,疾病活动评分更高,抗核小体抗体及抗核糖体P蛋白抗体阳性率升高;血清CG水平升高的SLE患者肾损伤和血液学异常发生率、抗dsDNA抗体和抗核小体抗体阳性率升高,疾病活动评分更高,C3、C4水平更低;血清PR3水平升高的SLE患者关节炎发生率更高(P<0.05)。结论:SLE患者血清NSPs水平较健康对照组显著升高,且与SLE疾病活动程度呈正相关,NSPs可能参与SLE疾病发生发展过程。Objective:To investigate expression and clinical significance of three main neutrophil serine proteases(NSPs NE,CG and PR3 in serum of systemic lupus erythematosus(SLE)patients.Methods:NE,CG and PR3 levels in serum of 66 patients with SLE and 39 healthy controls were measured by ELISA. Correlation between levels of NE,CG,PR3 and disease activity in patients with SLE was analyzed,and relationship between levels of NE,CG,PR3 and laboratory indexes and clinical manifestations in patients with SLE was analyzed.Results:Serum NE,CG and PR3 levels in SLE group were higher than those in healthy control group(P<0.05). Correlation analysis showed that serum NE,CG,PR3 levels in SLE patients were positively correlated with SLEDAI scores(r=0.420,0.269,0.475,P<0.05). SLE patients with increased serum NE levels had lower C4 levels,higher SLEDAI scores and higher positive rates of anti-nucleosomal antibodies and anti-ribosomal P protein antibodies. Incidence of renal damage and hematological abnormalities,positive rate of anti-dsDNA antibodies and anti-nucleosome antibodies were significantly higher,SLEDAI score was higher,and levels of C3 and C4 were lower in SLE patients with increased serum CG levels. SLE patients with increased serum PR3levels had a higher incidence of arthritis(P<0.05).Conclusion:Levels of serum NSPs in SLE patients are significantly higher than that in healthy controls,and is positively correlated with SLE disease activity. NSPs may be involved in occurrence and development of SLE.

关 键 词：系统性红斑狼疮 中性粒细胞丝氨酸蛋白酶 中性粒细胞弹性蛋白酶 组织蛋白酶G 蛋白酶3 

分 类 号：R593.24[医药卫生—内科学]