基于免疫信息学的SARS-CoV-2表位筛选及疫苗分子设计分析  

Epitopes screening and vaccine molecular design of SARS-COV-2 based on immunoinformatics

作　　者：邓辉雄 王革非(指导)[1] 李雁嫘 宋鑫利 谷李铭 李蕊(指导)[1] DENG Huixiong;WANG Gefei;LI Yanlei;SONG Xinli;GU Liming;LI Rui(Department of Microbiology and Immunology,Pathogen and Immunology Research Center,Shantou University Medical College,Shantou 515041,China)

机构地区：[1]汕头大学医学院微生物学与免疫学教研室病原与免疫学研究中心,汕头515041

出　　处：《中国免疫学杂志》2022年第7期838-847,共10页Chinese Journal of Immunology

基　　金：广东省自然科学基金项目(2021A1515012470);广东省教育厅项目(2020KZDZX1083);汕头市科技计划项目(汕府科[2020]16-2,汕府科[2020]53-51,汕府科[2021]88-STKJ2021197)资助。

摘　　要：目的:基于新型冠状病毒(SARS-CoV-2)全病毒或Spike蛋白作为免疫原可能存在的无关抗原表位干扰,筛选鉴定优势保护性抗原表位,构建候选多价表位疫苗。方法:以免疫信息学确定诱导主要中和抗体、激活细胞免疫应答的细胞保护性抗原表位,以Raptor X、trRosetta预测蛋白疫苗的二级、三级结构,并对候选蛋白疫苗进行二硫键的设计及对接分析,以C-ImmSim服务器模拟预测分析多表位疫苗的免疫原性和免疫应答。结果:实验筛选了Spike蛋白免疫原性强的B细胞表位,具有高保守性和人群覆盖度广的IFN-γ、IL-4阳性的Th表位及CTL表位,计算模拟验证了疫苗的免疫应答特性。结论:信息学分析结果初步显示候选表位疫苗具有良好的平衡体液免疫和细胞免疫应答能力。Objective:Based on SARS-Co V-2 whole virus or Spike protein as an immunogen may be interference with the epitopes of unrelated antigens.Constructed of candidate vaccine by screening and identifying the dominant protective epitopes.Methods:Immunoinformatics was used to identify the protective epitopes that induce the main neutralizing antibodies and activate the cellular immune response,Raptor X and trRosetta were used to predict the secondary and tertiary structures of the vaccine,and disulfide bond design and docking analysis were carried out for candidate protein vaccine,immunogenicity and immune response of multi-epitopes vaccine were analyzed using C-ImmSim server.Results:B cell epitopes on strong immunogenicity of Spike protein,highly conservative and wide coverage of population and positive IFN-γ,IL-4 of Th epitopes and CTL epitopes were selected,and the vaccine immune response characteristics were verified in silico.Conclusion:Results of immuneinformatics analysis preliminarily show that the candidate vaccine had a well-balanced on humoral and cellular immune response.

关 键 词：新冠病毒 SPIKE蛋白 抗原表位 平衡免疫 免疫信息学 

分 类 号：R392.1[医药卫生—免疫学]