人参皂苷G-Rh2通过调节脂质代谢与免疫改善小鼠代谢综合征  

Ginsenoside Rh2 ameliorates metabolic syndrome in high-fat diet induced obese mice by regulating lipid metabolism and immunity

作　　者：黄容容 张子君 解梦园 苟静 李晴 马坤 向明(指导)[2] HUANG Rongrong;ZHANG Zijun;XIE Mengyuan;GOU Jing;LI Qing;MA Kun;XIANG Ming(Department of Biological Medicine,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属协和医院药学部,武汉430030 [2]华中科技大学同济医学院药学院生物药学系,武汉430030

出　　处：《中国免疫学杂志》2022年第9期1030-1036,共7页Chinese Journal of Immunology

基　　金：国家自然科学基金面上项目(82071749);湖北省科技厅2020年重点研发计划一般项目(2020BHB019)。

摘　　要：目的:研究人参皂苷G-Rh2对高脂饮食(HFD)诱导小鼠代谢综合征(MS)的治疗效果和作用机制。方法:从小鼠体质量、胰岛素敏感性、血脂水平和肝脏功能方面评价G-Rh2对MS小鼠的治疗效果,广靶脂质代谢组学检测肝脏中脂质代谢物变化,ELISA检测血清中TNF-α、IL-6水平,流式细胞术检测脾脏、胸腺中CD4+T、CD8+T细胞、NK细胞和骨髓来源的抑制性细胞(MDSCs)数量。结果:G-Rh2明显减少MS小鼠的体质量和脂肪量,提高胰岛素敏感性,降低血脂总胆固醇(TC)、游离脂肪酸(NEFA)和低密度脂蛋白胆固醇(LDL-C)水平,防止肝脏脂质堆积。G-Rh2改变肝脏中脂质代谢模式,下调74种脂质代谢物,主要包括三酰甘油(TGs)和磷脂酰胆碱(PCs)两大类。G-Rh2降低血清中TNF-α和IL-6含量,平衡脾脏中CD4+T/MDSCs比例,减少肝脏中炎症细胞浸润。结论:G-Rh2对HFD诱导的小鼠MS有治疗作用,通过调节肝脏脂质代谢和抑制炎症双重途径实现。Objective:To explore the therapeutic effect and mechanism of G-Rh2 on metabolic syndrome(MS)in high-fat diet(HFD)induced obese mice.Methods:The therapeutic effect of G-Rh2 on MS mice was evaluated by body weight,insulin sensitivity,blood lipid level and liver function.The changes of lipid metabolites in liver were investigated by non-targeted lipid metabolomics technology.ELISA kits were used to detect TNF-α and IL-6 in serum.Immune cells CD4^(+)T,CD8^(+)T,NK cells and bone marrowderived suppressor cells(MDSCs)in spleen and thymus were detected by flow cytometer.Results:G-Rh2 significantly reduced body weight,and serum lipids level especially TC,NEFA,LDL-C of MS mice.It simultaneously alleviated insulin resistance and protected liver from steatohepatitis.Further,G-Rh2 down-regulated 74 lipid metabolites in liver,mainly including triglycerides(TGs)and phosphatidylcholine(PCs),which suggested G-Rh2 changed the liver metabolism pattern.On the other hand,G-Rh2 reduced the inflammatory cells infiltration in liver and TNF-α,IL-6 in serum.And it balanced the ratio of CD4^(+)T cells/MDSCs in spleen,which played an anti-inflammatory effect.Conclusion:G-Rh2 has a therapeutic effect on MS in HFD induced obese mice by regulating liver lipid metabolism and inhibiting regional inflammation.

关 键 词：代谢综合征 人参皂苷G-Rh2 脂质代谢 免疫 

分 类 号：R967[医药卫生—药理学]