趋化因子CXCL16对脓毒症小鼠生存率、组织损伤及炎症反应的影响  

Effects of chemokine CXCL16 on mortality,organ damage and inflammation in sepsis mice

作　　者：李佳熹 曹炬(指导)[1] LI Jiaxi;CAO Ju(The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学第一附属医院,重庆400016

出　　处：《中国免疫学杂志》2022年第9期1037-1041,1046,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金优秀青年基金项目(81722001)资助。

摘　　要：目的:探讨趋化因子CXCL16在脓毒症小鼠中的表达,组织损伤及炎症反应过程。方法:CLP诱导C57BL/6小鼠形成脓毒症模型,并收集血清,腹腔灌洗液及肝、肺、肾脏组织样本。记录小鼠生存情况;ELISA检测趋化因子CXCL16及IL-6、IL-8、IL-10、TNF-α和IFN-γ表达水平;血液分析仪计数炎症细胞;流式细胞术分选炎症细胞;病理切片观察组织损伤;Anti-CXCL16抗体蛋白治疗脓毒症并观察效果。结果:CXCL16在脓毒症小鼠血清、腹腔灌洗液、组织蛋白中表达水平显著升高(P<0.05)。与CLP+PBS组相比,CLP+CXCL16组小鼠生存率明显降低(P<0.05),中性粒细胞、巨噬细胞和淋巴细胞数量增加且促进炎症因子释放(P<0.05),肝、肺、肾组织损伤加重,肝肾功能血清指标ALT、AST、Urea和Creatinine升高(P<0.05)。与CLP+IgG组相比,Anti-CXCL6组死亡率降低,组织损伤减轻,肝肾功能血清指标降低(P<0.05),抗体治疗具有一定效果。结论:CXCL16在脓毒症小鼠中表达上调,其高表达与脓毒症免疫病理过程有关,CXCL16能够通过加重炎症反应促进脓毒症发生发展。Objective:To investigate the expression of chemokine CXCL16 in septic mice,tissue damage and inflammatoryprocess.Methods:CLP was performed on C57 BL/6 mice,abdominal infection was caused and sepsis model was done.Collectingserum,abdominal lavage fluid,liver,lung and kidney tissue samples after CLP.Mortality were recorded,expression levels of chemo-kine CXCL16 and IL-6,IL-8,IL-10,TNF-α,IFN-γ were detected by ELISA,inflammatory cells were sorted by flow cytometry,theinflammatory cells were sorted by flow cytometry,tissue damage was observed with HE staining in septic mice treated with CXCL16 recombinant protein,and therapeutic effects were observed in septic mice treated with CXCL16 antibody protein.Results:Expressionlevels of CXCL16 in serum,abdominal lavage fluid and tissue of sepsis mice were significantly increased(P<0.05).Compared with theCLP+PBS group,the survival rate of mice in the CLP+CXCL16 group was significantly reduced(P<0.05),the number of neutrophils,macrophages and lymphocytes was increased and the release of inflammatory factors was promoted(P<0.05),liver,lung and kidneytissue damage was aggravated,and serum indexes of ALT,AST,Urea and Creatinine of liver and kidney function were increased(P<0.05).Compared with the CLP+IgG group,the mortality rate of the anti-CXCL6 group was reduced,the tissue damage was reduced,the serum indexes of liver and kidney function were reduced(P<0.05),and the antibody treatment had a certain effect.Conclusion:Concentrations of CXCL16 are up-regulated in sepsis mice,and high expression of CXCL16 is related to the pathological process ofsepsis.CXCL16 can promote the development of sepsis by aggravating inflammatory response.

关 键 词：脓毒症 CXCL16 炎症 生存率 组织损伤 

分 类 号：R392.7[医药卫生—免疫学]