过敏性哮喘患者外周血循环T_(FR)细胞的变化及临床意义  

Alteration of peripheral blood circulating follicular regulatory T cells in patients with allergic asthma

作　　者：季拓 龚芳 吕蓓丽[2] 申卫红 白仲虎 金坚[1] JI Tuo;GONG Fang;LYU Beili;SHEN Weihong;BAI Zhonghu;JIN Jian(School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,无锡214122 [2]江南大学附属医院,无锡214122

出　　处：《中国免疫学杂志》2022年第9期1109-1113,共5页Chinese Journal of Immunology

基　　金：无锡市“双百”拔尖人才项目(BJ2020059);江苏省“333工程”科研项目(BRA2019152)资助。

摘　　要：目的:本研究旨在探讨过敏性哮喘患者外周血循环滤泡调节性T细胞(cT_(FR))比例变化及临床意义。方法:流式细胞仪检测25例过敏性哮喘患者及15例健康志愿者外周血cT_(FR)细胞的比例,流式细胞微球芯片捕获技术法检测血清细胞因子水平,并分析cT_(FR)细胞比例与过敏性哮喘临床实验室检测指标的相关性。结果:与健康对照组相比,过敏性哮喘患者外周血cT_(FR)细胞比例显著下降[(0.42±0.08)vs(0.91±0.12),P<0.01],血清IL-10表达水平显著降低(P<0.05)。cT_(FR)细胞比例与血清总IgE水平呈负相关(P<0.05),与第1秒用力呼气容积占预计值的百分比FEV1%呈正相关(P<0.05)。结论:外周血cT_(FR)和IL-10可能在过敏性哮喘免疫球蛋白E(IgE)异常增高的病理过程中起重要作用。Objective:This study aims to investigate the change and clinical significance of cT_(FR)cells in peripheral blood of patients with allergic asthma.Methods:Flow cytometer was used to detect the percentage of cT_(FR)from 25 allergic asthma patients and15 healthy controls.Cytometric bead array technology was used to detect serum cytokines levels.The correlation of cT_(FR)with clinical indicators was analyzed.Results:Compared with the healthy control group,the proportion of cT_(FR)cells in patients with allergic asthma decreased significantly[(0.42±0.08)vs(0.91±0.12),P<0.01],the level of serum IL-10 was significantly decreased(P<0.05).The proportion of cT_(FR)was negatively correlated with serum IgE(P<0.05)and positively correlated with FEV1%(P<0.05).Conclusion:Peripheral blood cT_(FR)and IL-10 may play an important role in the pathological process of abnormal immunoglobulin E(IgE)increase in allergic asthma.

关 键 词：过敏性哮喘 循环滤泡调节性T细胞(cT_(FR)) IL-10 

分 类 号：R392.8[医药卫生—免疫学]