浓缩生长因子纤维蛋白膜复合重组人表皮生长因子活性蛋白多肽修复口腔黏膜的等效损伤模型  

Repairing equivalent injury of oral mucosa with concentrated growth factor fibrin membrane combined with recombinant human epidermal growth factor active protein polypeptide complex

作　　者：何儒雅 刘芸伶 聂敏海 刘旭倩 He Ruya;Liu Yunling;Nie Minhai;Liu Xuqian(Department of Periodontics&Oral Mucosal Diseases,Affiliated Stomatology Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Luzhou Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属口腔医院牙周黏膜病科,四川省泸州市646000 [2]口颌面修复重建和再生泸州市重点实验室,四川省泸州市646000

出　　处：《中国组织工程研究》2023年第12期1848-1855,共8页Chinese Journal of Tissue Engineering Research

基　　金：四川省科技厅科技计划项目(2020YJ0387),项目负责人:刘旭倩;四川省泸州市人民政府-西南医科大学科技战略合作重点项目(2019LZXNYDZ10),项目负责人:刘旭倩;四川省干部保健普及应用项目(川干研2022-2101),项目负责人:刘旭倩。

摘　　要：背景:口腔黏膜的完整性和功能性对于隔绝外界刺激和抵御致病微生物的入侵有着极其重要的生物学意义。近年来,富自体浓缩生长因子与重组人表皮生长因子已然成为机体软组织损伤再生与修复领域中的两大研究热点。目的:探讨富自体浓缩生长因子联合重组人表皮生长因子复合纤维蛋白膜对于口腔黏膜等效损伤的修复价值。方法:分别制备富自体浓缩生长因子纤维蛋白膜与富自体浓缩生长因子纤维蛋白膜联合重组人表皮生长因子复合纤维蛋白膜。将人牙龈成纤维细胞与人永生化角质形成细胞分别接种于两种纤维蛋白膜上,组织形态学染色观察细胞增殖量。将24只BALB/c-裸鼠随机分为4组,A组为正常对照,B、C、D组在背部制作处于肌层之上的创面,C组在背部创面上覆盖富自体浓缩生长因子纤维蛋白膜,D组在背部创面上覆盖复合纤维蛋白膜,术后第3周末,通过组织形态学、免疫学评价各组创面的修复效果,生物力学检测各组修复组织的抗拉伸能力。结果与结论:①苏木精-伊红、SP免疫组化及免疫荧光染色显示,人牙龈成纤维细胞与人永生化角质形成细胞在两种纤维蛋白膜上生长良好,其中复合纤维蛋白膜上的细胞数量明显多于富自体浓缩生长因子纤维蛋白膜;②苏木精-伊红与Masson染色显示,B组表皮层基本愈合,棘细胞层厚度减低,胶原纤维量少且排列不连续;C组角细胞层、棘细胞层、基底细胞层出现不同程度的增厚,上皮钉突减少,胶原纤维粗大密集,包绕新生血管;D组表现与C组相似,但胶原纤维、成纤维细胞及新生血管数目明显增加;③修复组织拉应力峰值大小顺序为:A组>D组>C组>B组(P<0.05);④结果表明,相较于富自体浓缩生长因子纤维蛋白膜,富自体浓缩生长因子联合重组人表皮生长因子复合纤维蛋白膜不仅可促进人牙龈成纤维细胞与人永生化角质形成细胞的�BACKGROUND:The integrity and functionality of oral mucosa are of great biological significance for isolating external stimulus and resisting the invasion of pathogenic microorganisms.In recent years,concentrated growth factor and recombinant human epidermal growth factor have become two research hotspots in the field of soft tissue injury regeneration and repair.OBJECTIVE:To investigate the repair value of autogenous concentrated growth factor fibrin membrane combined with recombinant human epidermal growth factor active protein polypeptide complex for oral mucosa equivalent injury.METHODS:The autogenous concentrated growth factor and the autogenous concentrated growth factor fibrin membrane combined with recombinant human epidermal growth factor composite fibrin membrane were prepared respectively.Human gingival fibroblasts and human immortalized keratinocytes were cultured on concentrated growth factor fibrin membrane and concentrated growth factor+recombinant human epidermal growth factor fibrin membrane,respectively.The proliferation of cells on the membrane was compared by histological staining.The 24 BALB/c-nude mice were randomly divided into control group(group A)and back reconstruction group(group B:blank operation;Group C:concentrated growth factor fibrin membrane was covered after operation;Group D:concentrated growth factor+recombinant human epidermal growth factor fibrin membrane was covered after operation).The effect of wound repair in each group was evaluated by histomorphology and immunology,and the tensile resistance of repaired tissue in each group was detected by biomechanics at the weekend of the third week.RESULTS AND CONCLUSION:(1)Hematoxylin-eosin staining,SP immunohistochemistry,and immunofluorescence showed that cell proliferation of human gingival fibroblasts and human immortalized keratinocytes on concentrated growth factor+recombinant human epidermal growth factor fibrin membrane was significantly higher than that on concentrated growth factor fibrin membrane.(2)Hematoxylin-eosin stai

关 键 词：富自体浓缩生长因子 重组人表皮生长因子 口腔黏膜等效损伤 纤维蛋白膜 活性蛋白多肽 修复 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R781.5