槲皮素缓释系统对MC3T3-E1细胞成骨性能的影响  

Effects of quercetin sustained release system on osteogenic properties of MC3T3-E1 cells

作　　者：马子雨 张宾[2] 张云涛[2] 柳晓琳 卞志鸿 乔鲁卉 侯玉东 Ma Ziyu;Zhang Bin;Zhang Yuntao;Liu Xiaolin;Bian Zhihong;Qiao Luhui;Hou Yudong(Binzhou Medical College,Binzhou 256600,Shandong Province,China;Affiliated Hospital of Binzhou Medical College,Binzhou 256600,Shandong Province,China;Yantai Stomatological Hospital,Yantai 264010,Shandong Province,China;School of Stomatology,Binzhou Medical College,Yantai 264010,Shandong Province,China)

机构地区：[1]滨州医学院,山东省滨州市256600 [2]滨州医学院附属医院,山东省滨州市256600 [3]烟台市口腔医院,山东省烟台市264010 [4]滨州医学院口腔医学院,山东省烟台市264010

出　　处：《中国组织工程研究》2023年第12期1870-1876,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:槲皮素具有促成骨、抗过敏、抗炎症、抗氧化、抗病毒、降血压、抗血小板聚集等作用,但其稳定性及溶解性较差,不利于其功效的发挥和运用。目的:制备用于促进骨形成的槲皮素缓释纳米纤维支架,并考察该纳米纤维支架的体外释放机制及其对MC3T3-E1细胞成骨性能的影响。方法:以槲皮素、牛血清蛋白、壳聚糖为材料,采用改良去溶剂法制备壳聚糖包被的槲皮素纳米微球,和静电纺丝技术制备壳聚糖包被的槲皮素纳米微球;以槲皮素纳米微球、聚己内酯、聚乙二醇为原料,采用静电纺丝技术制备槲皮素缓释纳米纤维支架,表征纳米纤维的微观形貌、水接触角与体外药物释放。将聚己内酯/聚乙二醇纳米纤维支架与槲皮素缓释纳米纤维支架分别与MC3T3-E1细胞共培养,以单独培养的细胞为空白对照,检测3组细胞增殖与成骨分化能力。结果与结论:①透射电镜显示,槲皮素缓释纳米纤维支架表面光滑,直径不均匀,微球能够有效纺入静电纺丝支架中;扫描电镜显示,槲皮素缓释纳米纤维支架纤维不规则,交织成网状结构,形成大小不一的孔隙,无明显断裂;②槲皮素缓释纳米纤维支架的水接触角显著小于聚己内酯纳米纤维支架、聚己内酯/聚乙二醇纳米纤维支架;③槲皮素缓释纳米纤维支架前4 d释放的药物量达30%左右,后期药物释放逐渐平缓,1个月的药物释放量达到70%左右;④相较于空白对照组、聚己内酯/聚乙二醇纳米纤维支架,槲皮素缓释纳米纤维支架可促进MC3T3-E1细胞的增殖、碱性磷酸酶表达与钙盐沉积;⑤结果表明,槲皮素缓释纳米纤维支架具有良好的药物控释作用,可促进MC3T3-E1细胞的增殖与成骨分化。BACKGROUND:Quercetin plays a significant role in promoting osteogenesis,anti-allergy,anti-inflammatory,anti-oxidation,anti-virus,lowering blood pressure,and anti-platelet aggregation, but its stability and solubility are poor, which is not conducive to the exertion and application of its efficacy. OBJECTIVE: To prepare quercetin sustained-release nanofibers for promoting bone formation and to investigate in vitro release mechanism of quercetin sustained-release nanofibers and its effect on the osteogenic properties of MC3T3-E1 cells. METHODS: Quercetin, bovine serum albumin, and chitosan were used as materials. The quercetin sustained-release nanofibers coated with chitosan were prepared by improved solvent removal method and electrospinning technology. The quercetin sustained-release nanofibers, polycaprolactone, and polyethylene glycol were utilized as materials. The quercetin sustained-release nanofiber scaffolds were prepared using electrospinning to characterize microscopic morphology, water contact angle, and in vitro drug release of nanofibers. Polycaprolactone/polyethylene glycol nanofiber scaffolds and quercetin sustained-release nanofibers were cocultured with MC3T3-E1 cells. The cells cultured alone were taken as blank controls. The proliferation and osteogenic differentiation abilities of the three groups were detected. RESULTS AND CONCLUSION: (1) Transmission electron microscopy showed that quercetin sustained-release nanospheres had smooth surface and uniform diameter. The microspheres could be effectively spun into the electrospinning scaffold. Scanning electron microscopy showed that the quercetin sustained-release nanospheres were regular spheres. The nanofibers were irregularly interwoven into a network structure and formed pores of different sizes without obvious fracture. (2) The contact angle experiment showed that the water contact angle of quercetin sustained-release nanofiber group was significantly smaller than that of polycaprolactone group and polycaprolactone/polyethylene glycol group.

关 键 词：槲皮素 药物缓释 纳米颗粒 聚己内酯 聚乙二醇 静电纺丝 纳米纤维 成骨效应 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R783.1