运动训练老年骨质疏松大鼠骨量及骨微结构的变化  

Effects of exercise training on bone mass and bone microstructure in aged osteoporotic rats

作　　者：王金玲 黄夏荣 屈萌艰 黄福锦 尹林伟 钟培瑞 刘静 孙光华 廖阳 周君 Wang Jinling;Huang Xiarong;Qu Mengjian;Huang Fujin;Yin Lingwei;Zhong Peirui;Liu Jin;Sun Guanghua;Liao Yang;Zhou Jun(Rehabilitation Medicine Center,the First Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421001,Hunan Province,China;Department of Rehabilitation,the First Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421001,Hunan Province,China;Rehabilitation Laboratory,the First Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421001,Hunan Province,China)

机构地区：[1]南华大学衡阳医学院附属第一医院康复医学中心,湖南省衡阳市421001 [2]南华大学衡阳医学院附属第一医院康复医学科,湖南省衡阳市421001 [3]南华大学衡阳医学院附属第一医院康复医学实验室,湖南省衡阳市421001

出　　处：《中国组织工程研究》2023年第5期676-682,共7页Chinese Journal of Tissue Engineering Research

基　　金：湖南省自然科学基金面上项目(2020JJ4085),项目负责人:周君;湖南省卫健委重点项目(202103060198),项目负责人:周君;南华大学校级重点项目(USCKF201902K02),项目负责人:周君。

摘　　要：背景:运动训练有维持骨量、改善骨密度的作用,但其对老年骨质疏松的作用及可能机制尚不完全明确。目的:观察运动训练对老年骨质疏松的影响及可能的分子机制。方法:将16只24月龄老年SD大鼠按照随机抽样法分为老年模型组和运动训练组,每组8只;将8只3月龄青年SD大鼠作为青年对照组。3组大鼠在饲养笼中自由活动,其中运动训练组大鼠进行跑步机运动干预8周,训练结束后采用ELISA法检测血清骨源性碱性磷酸酶、Ⅰ型前胶原N-端前肽、抗酒石酸酸性磷酸酶和Ⅰ型胶原交联N-末端肽水平,双能X射线骨密度仪检测大鼠股骨和第4腰椎骨密度,Micro-CT检测并分析大鼠胫骨和第5腰椎的骨微结构及参数,反转录聚合酶链反应和Western blot检测股骨骨髓组织中骨形成蛋白2、Runt相关转录因子2、Osterix、过氧化物增殖激活受体γmRNA和蛋白的表达。结果与结论:①与老年模型组比较,运动训练组大鼠血清骨源性碱性磷酸酶和Ⅰ型前胶原N-端前肽水平升高(P<0.05),抗酒石酸酸性磷酸酶和Ⅰ型胶原交联N-末端肽水平降低(P<0.05);股骨骨密度升高(P<0.05);第4腰椎骨密度有上升的趋势,但无显著性差异(P>0.05);胫骨骨体积分数、骨小梁数量升高(P<0.05),骨小梁间隙降低(P<0.05),骨小梁厚度无明显变化(P>0.05);第5腰椎的骨体积分数、骨小梁数量、骨小梁厚度有升高的趋势,但无显著性差异(P>0.05);骨小梁间隙降低(P<0.05);股骨骨髓中骨形成蛋白2、Runt相关转录因子2与Osterix mRNA和蛋白表达升高(P<0.05);过氧化物增殖激活受体γmRNA和蛋白表达降低(P<0.05);②结果表明,运动训练可显著改善老年骨质疏松大鼠的骨量、骨微结构,促进成骨分化,抑制成脂分化,其机制可能与骨形成蛋白2/Runt相关转录因子2/Osterix信号通路有关。BACKGROUND:Exercise training can maintain bone mass and improve bone mineral density.However,its effect on senile osteoporosis and its possible mechanism are not clear.OBJECTIVE:To study the effects of exercise training on senile osteoporosis and to explore its possible molecular mechanism.METHODS:Sixteen Sprague-Dawley rats aged 24 months old were regarded as the model of senile osteoporosis and randomly divided into elderly model group(n=8)and exercise training group(n=8),while eight young Sprague-Dawley rats of 3 months old were randomly selected as young control group.All rats moved freely in their cages and those in the exercise training group underwent exercise training every day for 8 weeks.After 8 weeks of intervention,serum bone-specific alkaline phosphatase,type I procollagen amino-terminal peptide,tartrate-resistant acid phosphatase 5b and type I collagen aminoterminal peptide were detected using ELISA assay.Bone mineral density of the femur and the fourth lumbar vertebra were measured by dual energy X-ray absorptiometry,while bone microstructural parameters of the tibia and the fifth lumbar vertebra were determined by Micro-CT in each group.mRNA and protein expressions of bone morphogenetic protein 2,Runt-related transcription factor 2,Osterix,and peroxisome proliferator-activated receptorγin bone marrow of the femur were detected by reverse transcriptase polymerase chain reaction and western blot.RESULTS AND CONCLUSION:Compared with the elderly model group,the serum levels of bone-specific alkaline phosphatase and type I procollagen aminoterminal peptide(P<0.05),bone mineral density of the femur(P<0.05)but not that of the fourth lumbar vertebra(P>0.05),bone volume fraction and trabecular number of the tibia(P<0.05),and the mRNA and protein expression of bone morphogenetic protein 2,Runt-related transcription factor 2,and Osterix in the femur(P<0.05)were significantly increased in the elderly model group,while the serum levels of tartrate-resistant acid phosphatase 5b and type I collagen amino-termin

关 键 词：老年骨质疏松 运动训练 骨形成蛋白2 Runt相关转录因子2 OSTERIX 信号通路 骨形成 骨吸收 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R592