结扎不同部位输精管大鼠睾丸的生理功能  

Physiological function of the testis in rats undergoing different parts of vasectomy

作　　者：杨恒 郑丽英 刘志立 王勤章 郝志强 王敬珅 汪渊 李永乐 谭明辉 邹晓峰[2] 张国玺[2] 黄若辉 江波[2] 钱彪[1,2] Yang Heng;Zheng Liying;Liu Zhili;Wang Qinzhang;Hao Zhiqiang;Wang Jingshen;Wang Yuan;Li Yongle;Tan Minghui;Zou Xiaofeng;Zhang Guoxi;Huang Ruohui;Jiang Bo;Qian Biao(Department of Urology Surgery,the First Affiliated Hospital of Medical College in Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China;Department of Urology Surgery,the First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,Jiangxi Province,China)

机构地区：[1]石河子大学医学院第一附属医院泌尿外科,新疆维吾尔自治区石河子市832008 [2]赣南医学院第一附属医院泌尿外科,江西省赣州市341000

出　　处：《中国组织工程研究》2023年第5期720-725,共6页Chinese Journal of Tissue Engineering Research

基　　金：赣州市2020年指导性科技计划项目(GZ2020ZSF060),项目负责人:黄若辉;赣南医学院第一附属医院院级科技计划项目(YJYB202062),项目负责人:黄若辉;石河子大学成果转化与技术推广项目(CGZH201810),项目负责人:钱彪;基于院、县“博士专家工作站”模式下的男科显微微创技术的推广应用(SKJP420203656),项目负责人:钱彪;石河子大学科研项目(ZZZC201823A),项目负责人:刘志立。

摘　　要：背景:输精管结扎是目前有效的男性节育方法,而人们主要关注的是输精管结扎后的临床并发症。目的:研究不同手术方式的输精管结扎对大鼠睾丸生理功能的影响。方法:12只雄性SD大鼠适应性喂养1周后随机分为4组,每组3只,分别为阴性对照组、假手术组、手术组和传统组。手术组大鼠行双侧输精管斜行切断,近端结扎,远端旷置;传统组行双侧输精管斜行切断,两端均结扎;假手术组游离双侧输精管,不结扎;阴性对照组不做特殊处理。术后3个月注射美蓝观察造模情况。收集睾丸行苏木精-伊红染色常规病理检测;免疫组化检测睾丸组织Bax、Bcl-2、3βHSD和附睾蛋白4的表达;TUNEL法检测睾丸组织细胞凋亡情况;ELISA/生化检测血清白细胞介素1、睾酮水平。结果与结论:①苏木精-伊红染色结果:与假手术组相比,传统组和手术组大鼠睾丸肿胀,质地较硬,传统组睾丸无菌性炎症的发生率高于阴性对照组,手术组的睾丸均未见到精子性肉芽肿的形成,传统组中的睾丸的组织结构则有显著改变;②TUNEL检测结果:与阴性对照组和假手术相比,手术组和传统组大鼠的细胞凋亡升高,其中手术组比传统组细胞凋亡更少;③免疫组化检测结果:与阴性对照组相比,假手术组Bax表达差异无显著性意义,手术组和传统组Bax表达升高,其中传统组更高;手术组和传统组的Bcl-2表达下降,其中传统组更低;与阴性对照组和假手术组相比,手术组和传统组大鼠睾丸组织3βHSD和附睾蛋白4蛋白的表达更低,其中手术组和传统组比较差异无显著性意义;④ELISA/生化检测结果:与阴性对照组相比,手术组大鼠血清白细胞介素1、睾酮水平升高(P<0.05),传统组白细胞介素1、睾酮水平下降(P<0.05);⑤结果表明,盆部输精管结扎对大鼠睾丸生理功能影响较小。BACKGROUND:Vasectomy is currently an effective method of male birth control,and the main concern is clinical complications after vasectomy.OBJECTIVE:To study the effects of different surgical methods of vasectomy on physiological function of the testis in rats.METHODS:After 1-week adaptive feeding,12 male Sprague-Dawley rats were randomly selected and randomly divided into 4 groups with 3 rats in each group,including negative control group,sham-operation group,operation group and traditional group.The rats in the operation group were subjected to bilateral oblique vasectomy,the proximal end of the vas deferens was ligated and the distal end was separated but not ligated.The rats in the traditional group underwent bilateral oblique vasectomy,with both ends of the vas deferens ligated.In the sham-operation group,the both ends of the vas deferens were separated without ligation.The negative control group had no special treatment.Three months after the operation,methylene blue was injected and observed.After anesthesia,the rats were sacrificed and their testicles were collected for routine pathological examination using hematoxylin-eosin staining.Testicular tissue was collected and the expressions of Bax,Bcl-2,3βHSD and human epididymal protein 4 were detected by immunohistochemistry.Apoptosis in testicular tissue was detected by TUNEL assay.Serum interleukin-1 and testosterone levels were detected using ELISA/biochemical detection.RESULTS AND CONCLUSION:Hematoxylin-eosin staining:compared with the sham operation group,the testis was swollen and hard in texture in the traditional and operation groups;the incidence of testicular aseptic inflammation in the traditional group was higher than that in the negative control group;no spermatogenic granuloma was found in the testis of the operation group and the histological structure of the testis in the traditional group was remarkably changed.TUNEL detection:Compared with the negative control and sham operation groups,apoptosis was increased in the operation and tradition

关 键 词：输精管结扎 不同手术方式 睾丸 生理功能 附睾蛋白4 大鼠 

分 类 号：R692.4[医药卫生—泌尿科学] R446[医药卫生—外科学] R318[医药卫生—临床医学]