大豆维生素E及组分含量特异种质发掘鉴定  

Exploration and Identification of Soybean Special Germplasm of Vitamin E and Its Components

作　　者：上官文秀 王敏[1] 岳爱琴[1] 赵晋忠[2] 牛景萍 张永坡[2] 高春艳[2] 张武霞 郭数进[1] 王鹏[1] 王利祥 杨婷婷 张海生 杜维俊[1] SHANGGUAN Wenxiu;WANG Min;YUE Aiqin;ZHAO Jinzhong;NIU Jingping;ZHANG Yongpo;GAO Chunyan;ZHANG Wuxia;GUO Shujin;WANG Peng;WANG Lixiang;YANG Tingting;ZHANG Haisheng;DU Weijun(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Department of Basic Courses,Shanxi Agricultural University,Taigu 030801,China;College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学基础部,山西太谷030801 [3]山西农业大学生命科学学院,山西太谷030801

出　　处：《山西农业科学》2022年第7期979-986,共8页Journal of Shanxi Agricultural Sciences

基　　金：山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-3-8);山西农业大学育种工程项目(YZGC096);山西省自然基金项目(201901D111225);山西农业大学农学院育种工程重点培育专项(YZ2021-05)。

摘　　要：为筛选维生素E及组分含量特异种质资源,研究以327份大豆种质资源为材料,采用改良的高效液相色谱法(HPLC),对大豆籽粒中的维生素E及其组分α-生育酚、γ-生育酚、δ-生育酚的含量进行测定。结果表明,利用改良的高效液相色谱法(HPLC)测定大豆维生素E含量精密度高,可靠性好;大豆种质资源中,维生素E及各生育酚含量的变异系数均大于20%。α-生育酚、总维生素E的含量呈现正偏态分布,γ-生育酚、δ-生育酚的含量呈正态分布;α-生育酚、γ-生育酚、δ-生育酚和总维生素E的含量之间都存在极显著相关关系。对种质资源各组分进行聚类分析,结果筛选出富含维生素E的大豆品种34个。热图分析表明,富含维生素E的不同种质各生育酚组分含量存在差异,筛选出α-生育酚含量较高的2份种质资源东北黑豆和山东平邑豆,γ-生育酚含量较高的3份种质资源SCS-9299、辽豆22和朔州平鲁双色豆,3种生育酚含量都较高的5份种质资源富黑2号、黑珍珠、于秀芝翠绿、特大粒黑皮绿豆、青仁黑一号。In order to screen special germplasm resources of vitamin E component, in this study, 327 soybean germplasm resources were taken as materials, the content of vitamin E and its components α-tocopherol, γ-tocopherol, and δ-tocopherol in the seeds of 327 soybean germplasm resources were determined by improved high performance liquid chromatography(HPLC).The results showed that the improved HPLC method was accurate and reliable for the determination of vitamin E in soybean.The variation coefficients of vitamin E and tocopherol contents in soybean germplasm resources were greater than 20%. The contents of α-tocopherol and total vitamin E were positively skewed, while γ and δ-tocopherol were normally distributed. The contents of α-tocopherol, γ-tocopherol, δ-tocopherol, and total vitamin E were significantly correlated. Thirty-four soybean varieties rich in vitamin E were screened by cluster analysis. Heat map analysis showed that different contents of tocopherol components of different germplasm rich in vitamin E had difference. Two germplasm resources with high content ofα-tocopherol, DongBei Black Bean and Shandongpingyi Bean, were selected. Three germplasm resources with high content ofγ-tocopherol, SCS-9299, Liaodou 22 and Shuozhoupinglu Double-color Bean were screened. Five germplasm resources with higher contents of all three tocopherols, Fuhei 2, Heizhenzhu, Yuxiuzhicuilu, Extra-large Black Testa Mung, and Qingrenhei 1were selected.

关 键 词：大豆 维生素E 改良的高效液相色谱法 检测评价 特异种质 

分 类 号：S565.1[农业科学—作物学]