糜子芽苗期对干旱胁迫的响应及抗旱种质鉴定  

Response of Broomcorn Millet to Drought Stress at Bud and Seedling Stages and Identification of Drought Resistant Germplasm

作　　者：程冀南 李玉露 王宇卓 Santra Dipak K 王瑞云[2,4] CHENG Ji'nan;LI Yulu;WANG Yuzhuo;SANTRA Dipak K;WANG Ruiyun(Shennong Zhihua Biotechnology(Shanxi)Co.,Ltd.,Changzhi 046200,China;College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Panhandle Research and Extension Center,University of Nebraska-Lincoln,Scottsbluff 69361,USA;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan 030031,China)

机构地区：[1]神农智华生物科技(山西)有限公司,山西长治046200 [2]山西农业大学农学院,山西太谷030801 [3]内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心,美国内布拉斯加州69361 [4]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031

出　　处：《山西农业科学》2022年第7期987-995,共9页Journal of Shanxi Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-06-13.5-A16);国家自然科学基金项目(31271791);山西省自然科学基金项目(201901D11126);山西省现代农业产业技术体系建设(杂粮)项目(2022-03)。

摘　　要：为了了解内蒙古现存糜子种质资源的抗旱性,研究糜子在PEG-6000模拟干旱胁迫下的抗旱表现,挖掘优异抗旱种质并筛选抗旱鉴定指标,以33份来源于内蒙古的糜子核心种质资源为试验对象,在PEG-6000模拟干旱胁迫下,利用相关性分析、主成分分析、平均值-标准差逐级分级法和聚类分析等方法评估了材料芽期、苗期的抗旱性。结果表明,干旱胁迫下,芽期和苗期各指标较对照组均有下降趋势,糜子芽期比苗期更易受干旱胁迫,发芽势对干旱胁迫反应最敏感,变异系数为380.4%,苗期各指标变异系数均在13.2%~67.4%。糜子多数抗性指标间呈显著或极显著相关,其中相对苗长与相对苗鲜质量之间的相关性最大,相对根长与相对根干质量的相关性最小。对33份核心种质进行了芽苗期抗旱性鉴定和分类分析,结果鉴定出乌克兰黍(00006438)和伊盟良56-2黍(00000684)芽期抗旱性最强;准旗酱黄黍(00000650)、和林红糜子(00002160)、乌克兰黍(00006438)、8311/4/5(00005884)、伊选大红黍(00002473)和达旗青糜子(00002385)等6份种质苗期抗旱性好,乌克兰黍(00006438)芽期和苗期抗旱性能均突出。In order to understand the drought resistance of existing broomcorn millet germplasm resources in Inner Mongolia, study the drought resistance performance of broomcorn millet under PEG-6000 simulated drought stress, excavate excellent drought resistant germplasm, and screen drought resistance identification indexes, 33 broomcorn millet core germplasm resources from Inner Mongolia were used as experimental subjects. Under PEG-6000 simulated drought stress, the drought resistance of bud and seedling stages of materials was evaluated by correlation analysis, principal component analysis, averagestandard deviation grading method, and cluster analysis. The results showed that under drought stress, the indexes at bud stage and seedling stage showed a downward trend compared with those of the control group. The bud stage was more vulnerable to drought stress than the seedling stage. The germination potential was the most sensitive to drought stress, and the coefficient of variation was 380.4%. The coefficient of variation of each index at seedling stage was 13.2%-67.4%. Most of the resistance indexes of broomcorn millet showed significant or extremely significant correlations, among which the correlation between relative seedling length and relative seedling fresh weight was the largest, and the correlation between relative root length and relative root dry weight was the smallest. Drought resistance identification and classification analysis of 33 core germplasms at bud and seedling stages were carried out. Drought resistance at bud stage of Ukrainian broomcorn millet(00006438) and Yimengliang 56-2 broomcorn millet(00000684) were the strongest. Six germplasms, such as Zhunqi Jianghuang broomcorn millet(00000650), Helinhong broomcorn millet(00002160), Ukraine broomcorn millet(00006438), 8311/4/5(00005884),Yixuandahong broomcorn millet(00002473), and Daqiqing broomcorn millet(00002385), had good drought resistance at seedling stage, and Ukraine broomcorn millet(00006438) had outstanding drought resistance at both bud sta

关 键 词：糜子 PEG-6000胁迫 芽苗期 相关性分析 主成分分析 抗旱性 

分 类 号：S516[农业科学—作物学]