Candida albicans aspects of binary titanium alloys for biomedical applications  

作　　者：Shuyang Chen James K.H.Tsoi Peter C.S.Tsang Yeong-Joon Park Ho-Jun Song Jukka P.Matinlinna 

机构地区：[1]Dental Materials Science,Division of Applied Oral Sciences&Community Dental Care,Faculty of Dentistry,The University of Hong Kong,Prince Philip Dental Hospital,34 Hospital Road,Hong Kong SAR,People’s Republic of China [2]Department of Prosthodontics,Tianjin Stomatological Hospital,No.75,Dagu Road,Heping District,Tianjin 300041,People’s Republic of China [3]Division of Restorative Dental Sciences,Faculty of Dentistry,The University of Hong Kong,Prince Philip Dental Hospital,34 Hospital Road,Hong Kong SAR,People’s Republic of China [4]and 4 Department of Dental Materials and MRC for Hard-tissue Biointerface,School of Dentistry,Chonnam National University,Gwangju 61186,Republic of Korea

出　　处：《Regenerative Biomaterials》2020年第2期213-220,共8页再生生物材料（英文版）

基　　金：We knowledge Chonnam National University(NRF No.2011-0030121),Korea for their donation of the Ti-alloy and cp-Ti discs.

摘　　要：Titanium and its alloys are widely used in biomedical devices,e.g.implants,due to its biocompati-bility and osseointegration ability.In fact,fungal(Candida spp.)infection has been identified as one of the key reasons causing the failure of the device that is inevitable and impactful to the society.Thus,this study evaluated the surface morphology,surface chemical composition and Candida albicans adhesion on specimens of 16 binary Tialloys(-5 wt%of any one of the alloy elements:Ag,AI,Au,Co,Cr,Cu,Fe,In,Mn,Mo,Nb,Pd,Pt,Sn,V and Zr)compared with cp-Ti,targeting to seek for the binary Ti-alloys which has the lowest C.albicans infection.Candida albicans cultures were grown on the specimens for 48 h,and colony forming units(CFUs)and real-time polymerase chain reaction(RT-PCR)were used to evaluate the biofilm formation ability.Scanning electron mi-croscopy and confocal laser scanning microscopy confirmed the formation of C.albicans biofilm on all specimens'surfaces,such that CFU results showed Ti-Mo,Ti-Zr,Ti-AI and Ti-V have less C.albicans formed on the surfaces than cp-Ti.RT-PCR showed Ti-Zr and Ti-Cu have significantly higher C.albicans DNA concentrations than Ti-AI and Ti-V(P<0.05),whereas Ti-Cu has even showed a statistically higher concentration than Ti-Au,Ti-Co,Ti-In and Ti-Pt(P<0.05).This study confirmed that Ti-Mo,Ti-Zr,Ti-AI and Ti-V have lower the occurrence of C.albicans which might be clinically advantageous for medical devices,but Ti-Cu should be used in caution.

关 键 词：TITANIUM binary titanium alloy C.albicans fungal infection medical devices 

分 类 号：R318[医药卫生—生物医学工程]