响应曲面法优化大花红景天总黄酮的提取工艺  

Optimization of Extraction Process of Total Flavonoids from Rhodiola crenulate

作　　者：刘锦潘 孙羽珂 何正军[1] 赵文吉[1] 贾国夫[1] 敬璞[3] 孙继林 陈宇航[2] 梁提松 童琪[1] Liu Jinpan;Sun Yuke;He Zhengjun;Zhao Wenji;Jia Guofu;Jing Pu;Sun Jilin;Chen Yuhang;Liang Tisong;Tong Qi(Sichuan Academy of Grassland Sciences,Chengdu 611743,China;School of Pharmacy,Chengdu Medical College,Chengdu 610500,China;School of Agriculture&Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Sichuan Fuzheng Pharmaceutical Co.,Ltd,Chengdu 611730,China)

机构地区：[1]四川省草原科学研究院,四川成都611743 [2]成都医学院,四川成都610500 [3]上海交通大学,上海200240 [4]四川辅正药业股份有限公司,四川成都611730

出　　处：《草学》2022年第3期58-64,83,共8页Journal of Grassland and Forage Science

基　　金：四川省科技计划项目(2019YFSY0010);四川省中医药科学技术研究专项项目(2019PC005);阿坝州科技条件能力建设和技术示范推广项目(19TJNLJS0014);四川省级大学生创新创业训练计划项目(202113705016)共同资助。

摘　　要：目的:优化大花红景天总黄酮的提取工艺,提高大花红景天总黄酮提取效率。方法:采用超声辅助提取法提取大花红景天药材中有效成分,在单因素试验基础上,选取乙醇浓度、提取温度、提取时间3个因素进行Box-Behnken中心组合设计,以总黄酮提取率为指标,利用响应曲面法优化大花红景天总黄酮提取的最佳工艺。结果:大花红景天总黄酮最佳提取工艺条件为超声提取时间15min,乙醇浓度25%,超声提取温度40℃,在此条件下提取率为23.89%。结论:该模型回归性良好,试验拟合程度高、经验证该方法准确可靠,可用于大花红景天总黄酮的提取,为大花红景天资源化学的开发利用提供科学依据。The extraction process of Rhodiola crenulata(HooK. f. et.Thoms) H. Ohba was optimized by response surface optimization ultrasonic assisted method, to provide scientific basis for optimizing the extraction of effective components from R.crenulata. Methods: On the basis of single factor experiment, the effects of ethanol concentration, ultrasonic temperature and ultrasonic time on the extraction yield amount of total flavonoids in R.crenulata were studied by response surface test. Results: The results showed that the optimal extraction parameters of total flavonoids were: Ethanol concentration of 25%,ultrasonic time was 15 min, ultrasonic temperature 40℃. Under this condition, the extraction rate is 23.89% Conclusion: The response surface optimization ultrasonic-assisted extraction method effectively improved the extraction yield amount of flavonoids from R.crenulata,the model has good regression and high fitting degree, to provide effective extraction method basis for the development and utilization of R.crenulata.

关 键 词：大花红景天 总黄酮 超声辅助提取法 响应面分析 

分 类 号：S540.35[农业科学—作物学]