泥鳅维氏气单胞菌的毒力基因与耐药特性分析  

Virulence Genes and Antibiotic Resistance Characteristics of Pathogenic Aeromonas veronii Isolated from Loach Misgurnus anguillicaudatus

作　　者：韩语 潘纪汶 王昕 杨诺 郭桂英 李迁[3] 曾纪锋[4] 郑继平[1] HAN Yu;PAN Jiwen;WANG Xin;YANG Nuo;GUO Guiying;LI Qian;ZENG Jifeng;ZHENG Jiping(School of Life Sciences,Hainan University,Haikou 570228,China;School of Science,Hainan University,Haikou 570228,China;Network and Technology Center,Hainan University,Haikou 570228,China;School of Animal Science and Technology,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学生命科学学院,海南海口570228 [2]海南大学理学院,海南海口570228 [3]海南大学网络与技术中心,海南海口570228 [4]海南大学动物科技学院,海南海口570228

出　　处：《水产科学》2022年第4期564-572,共9页Fisheries Science

基　　金：国家自然科学基金资助项目(32060131,32060788);海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC084);海南省自然科学基金资助项目(321MS007).

摘　　要：为查明海南和广东地区维氏气单胞菌的毒力基因携带率及耐药情况,从两个地区的6个泥鳅养殖场的46尾泥鳅中,采集肾脏、肝脏、皮肤溃疡部位等样品进行细菌的分离,通过形态与生理生化鉴定、gyrA管家基因序列分析筛选到20株维氏气单胞菌(海南源7株、广东源13株),并对其毒力基因、耐药基因和抗生素药物敏感性进行检测。结果显示:20株分离的维氏气单胞菌的形态特征和生理生化特性与《常见细菌系统鉴定手册》中的维氏气单胞菌一致;gyrA管家基因序列均与GenBank数据库中维氏气单胞菌具有90%~99%的同源性。在20株维氏气单胞菌中,气溶素、肠毒素、蛋白酶等毒力基因检出率高,其中气溶素基因和鞭毛蛋白基因检出高达100%,但热稳定肠毒素基因未检出;喹诺酮类以及磺胺类药物耐药基因检出率均为100%,而酰胺醇类、大环类酯类和林可霉素类药物耐药基因均未检测到。这20株维氏气单胞菌对甲氧苄啶、红霉素、诺氟沙星、氨苄西林和林可霉素等药物的耐受率达100%,对氨曲南、庆大霉素和头孢噻肟等5种药物具有高度敏感性。试验结果可为泥鳅养殖生产中维氏气单胞菌的防控提供参考。In order to understand the virulence gene carrier rate and drug resistance of Aeromonas veronii in Hainan and Guangdong provinces,the kidney,liver,and skin ulcers were collected from 46 samples of loach Misgurnus anguillicaudatus from 6 farms in the two regions.The samples were isolated from the bacteria,and 20 strains of A.veronii(7 from Hainan province and 13 from Guangdong province)were screened through morphological,physiological and biochemical identification and gyrA(gyrase A subunit)housekeeping gene sequence analysis,and their virulence genes and drug resistance genes and antibiotic susceptibility were tested.The results showed that the morphological,physiological and biochemical characteristics of the 20 isolated A.veronii were consistent with those reported in the"Handbook of Common Bacteria Identification";the gyrA housekeeping gene sequence was consistent with that in the GenBank database.The spores had 90%-99%homology.Among the 20 strains of A.veronii,the detection rate of virulence genes such as aerolysin,enterotoxin,and protease was high in which the aerolysin gene and flagellin gene were detected as high as 100%,but the heat-stable enterotoxin gene was not detected;the detection rate of quinolone and sulfonamide drug resistance genes was 100%,while the amide alcohol,macrocyclic lipid and lincomycin drug resistance genes were not detected.The tolerance rate of erythromycin,norfloxacin,ampicillin and lincomycin and trimethoprim was 100%,highly sensitive to five drugs including aztreonam,gentamicin and cefotaxime.The findings provide a reference for the prevention and control of A.veronii in the production of loach M.anguillicaudatus.

关 键 词：泥鳅 维氏气单胞菌 毒力基因 耐药基因 药物敏感性 

分 类 号：S917.1[农业科学—水产科学]