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作 者:刘苏萱 宋伦[2] 胡超魁[2] 王昆[2] 刘印 张明亮[1] 董少杰 LIU Suxuan;SONG Lun;HU Chaokui;WANG Kun;LIU Yin;ZHANG Mingliang;DONG Shaojie(Dalian Ocean University,Dalian 116023,China;Key Laboratory of Marine Biological Resources and Ecology,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;Key Laboratory of Aqua-Ecology and Aquaculture of Tianjin,College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China)
机构地区:[1]大连海洋大学,辽宁大连116023 [2]辽宁省海洋水产科学研究院,辽宁省海洋生物资源与生态学重点实验室,辽宁大连116023 [3]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384
出 处:《水产科学》2022年第4期645-651,共7页Fisheries Science
基 金:辽宁省兴辽英才计划项目(XLYC1907109);辽宁省重点研发计划项目(2018228004);辽宁省海洋与渔业科研项目(201828,201824);天津市重点研发计划项目(19YFZCSN00070);大连市杰出青年科技人才项目(2019RJ09).
摘 要:采用高通量测序—分子鉴定分级技术,研究辽东湾海域微藻粒级结构。研究结果显示,辽东湾4个季节主要以小粒级微藻为主,优势种为抑食金球藻、细小微胞藻、微拟球藻属、葡萄藻属。春、夏、秋、冬季小粒级微藻生物量占比分别高达(51±14)%、(43±14)%、(49±6)%、(42±4)%,中粒级微藻生物量占比分别为(29±7)%、(34±15)%、(24±5)%、(42±4)%,大粒级微藻生物量占比分别为(21±10)%、(23±13)%、(27±3)%、(17±4)%。辽东湾微藻饵料供饵力主要为春、夏季贡献,春季供饵力较高区域主要分布在辽东湾顶部及中部,夏季主要分布在辽东湾东南侧和西侧海域。辽东湾整个海域实际可承载贝类养殖容量:春季为155g/m^(2)、夏季为203g/m^(2)、秋季为48g/m^(2)、冬季为33g/m^(2)。本研究结果可为滤食性贝类科学增养殖提供技术指导。The feeding power of microalgae food in Liaodong Bay was studied by high-throughput sequencing-molecular identification and grading technique.The results showed that small-sized microalgae dominated Liaodong Bay in four seasons,with the dominant species of Aureococcus anophagefferens,Microcystis pusilla,Nannochloris sp.and Botrytis sp..In spring,summer,autumn and winter,the biomass of microalgae of small size was as high as(51±14)%,(43±14)%,(49±6)%and(42±4)%,the biomass of medium size was(29±7)%,(34±15)%,(24±5)%and(42±4)%,and the biomass of large size was(21±10)%,(23±13)%,(27±3)%and(17±4)%,respectively.The feeding power of microalgae in Liaodong Bay was mainly contributed by spring and summer.The areas with higher feeding power in spring were mainly distributed in the upper and middle of Liaodong Bay,while in summer they were mainly distributed in the southeast and west waters of Liaodong Bay.The actual carrying capacity of shellfish in Liaodong Bay was 155 g/m^(2) in spring,203 g/m^(2) in summer,48 g/m^(2) in autumn and 33 g/m^(2) in winter.The finding can provide technical guidance for scientific culture and multiplication of filter-feeding shellfish.
关 键 词:贝类饵料 粒级结构 高通量测序—分子鉴定分级技术 辽东湾
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