亚硝态氮对虾肝肠胞虫感染脊尾白虾的影响  

Effect of Nitrite Nitrogen on Infection of Parasite Enterocytozoon hepatopenaei in Ridgetail White Prawn Exopalaemon carinicauda

作　　者：胡吉卉 段健诚 高阳 陈姝含 张庆起 牟华 赖晓芳 高焕 HU Jihui;DUAN Jiancheng;GAO Yang;CHEN Shuhan;ZHANG Qingqi;MU Hua;LAI Xiaofang;GAO Huan(Key Laboratory of Marine Biotechnology of Jiangsu Province,College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Lianyungang Ganyu Jiaxin Aquatic Products Development Co.,Ltd.,Liangyungang 222100,China;Jiangsu Marine Biological Industry Technology Collaborative Innovation Center,Lianyungang 222005,China;Jiangsu Provincial Platrorm for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [2]连云港赣榆佳信水产开发有限公司,江苏连云港222100 [3]江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [4]江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《水产科学》2022年第4期664-669,共6页Fisheries Science

基　　金：国家重点研发计划项目(2018YFD0901302);江苏省优势学科建设工程项目(PAPD);江苏省研究生科研与实践创新计划项目(SJCX20-1272).

摘　　要：亚硝态氮是诱发虾类暴发性疾病的重要因素之一。为探究亚硝态氮胁迫对脊尾白虾生长状况及体内虾肝肠胞虫携带量的影响,设置0.008、3.000、6.000、9.000、12.000mg/L5个亚硝态氮质量浓度试验组,跟踪测定3周内脊尾白虾的生长指标和虾肝肠胞虫载量的变化。试验结果显示,脊尾白虾存活率、体长增量和体质量增加率均随着亚硝态氮质量浓度的升高呈先升后降的趋势,其中亚硝态氮6.000mg/L组达最大值,存活率、体长增量和体质量增加率平均值分别为29%、0.8cm和35.33%。脊尾白虾虾肝肠胞虫的载量随亚硝态氮质量浓度的升高呈现先降后升的趋势,在6.000mg/L组达到最低值,为4.4×103拷贝/mg。试验结果表明,6.000mg/L的亚硝态氮可抑制虾肝肠胞虫的传播,进而对脊尾白虾生长有一定的促进作用,提高感染虾肝肠胞虫的脊尾白虾的存活率和养殖产量。Nitrite nitrogen is one of the important factors to induce the outbreak disease of shrimp.In order to investigate the effects of nitrite stress on the growth status and the carrying capacity of parasite Enterocytozoon hepatopenaei in ridgetail white prawn Exopalaemon carinicauda,five experimental groups with different nitrite nitrogen concentrations(0.008,3.000,6.000,9.000 and 12.000 mg/L)were set up to determine the changes in growth indices and EHP load of ridgetail white prawn in three weeks.The results showed that the survival rate,body length and body weight gain rate of ridgetail white prawn were increased first and then decreased with the increase in nitrite nitrogen concentration.The maximum value was reached in nitrite nitrogen 6.000 mg/L group.The increment of average survival rate,body length and body weight gain rate were 29%,0.8 cm and 35.33%,respectively.With the increase in nitrite nitrogen concentration,the EHP load of ridgetail white prawn was decreased first and then increased,and reached the lowest value in 6.000 mg/L group,which was 4.4×103 copies/mg.It was found that 6.000 mg/L nitrite nitrogen led to inhibit the transmission of EHP and to promote the growth of ridgetail white prawn.The purpose of this study is to explore the effect of nitrite nitrogen on the transmission rate of EHP,and help to control the concentration of nitrite reasonably in order to inhibit the spread of EHP,so as to improve the survival rate and breeding yield of ridgetail white prawn infected with Enterocytozoon hepatopenaei.

关 键 词：脊尾白虾 虾肝肠胞虫 亚硝态氮 生长状况 载量 

分 类 号：S945.4[农业科学—水产养殖]