维生素C碳点对水产养殖主要病原菌的抗菌性能及其生物相容性  

Antibacterial activity of vitamin C-based carbon dots against main pathogenic bacteria in aquaculture and its biocompatibility

作　　者：何稳 田丹阳 孔令娇 菅攀阳 方洁 孙丙耀[1] 宋学宏[1] HE Wen;TIAN Danyang;KONG Lingjiao;JIAN Panyang;FANG Jie;SUN Bingyao;SONG Xuehong(School of Biology and Basic Medical Sciences,Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《水产学报》2022年第5期848-856,共9页Journal of Fisheries of China

基　　金：国家自然科学基金(31772896);江苏高校优势学科建设工程资助项目。

摘　　要：为了验证维生素C碳点对水生动物病原菌的抑制效率,实验采用3倍梯度稀释法检测了维生素C碳点对嗜水气单胞菌、副溶血性弧菌、灿烂弧菌、诺卡氏菌等12种常见水产病原菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),在此基础上,测定了其对这12种水产病原菌的抑菌动力学曲线。此外,基于纳米材料可能存在的生物相容性等问题,实验采用MTT法检测了维生素C碳点对草鱼肾细胞的细胞毒性,并以斑马鱼胚胎为测试对象,测定其经维生素C碳点暴露后的胚胎毒性。结果显示,维生素C碳点对所监测的12种水产病原菌均有显著的抑制作用,MIC为20.6~61.7μg/mL,基本达到与抗生素氟苯尼考同等的抗菌作用。维生素C碳点在MIC范围内胚胎和细胞存活率可接近100%,与对照组无差异。研究表明,维生素C碳点对水产主要病原菌有良好的杀菌和抗菌效果,具有较好的生物相容性,在水产养殖细菌性病害防治上具有潜在的开发应用前景。Vitamin C-based carbon dots are a new class of nanomaterials with antibacterial properties,but their antibacterial activities against aquatic pathogenic bacteria have not been verified.For this reason,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of V;-CDs against 12 aquatic pathogenic bacteria such as Aeromonas hydrophila,Vibrio parahaemolyticus,V.splendens and Nocardia sp.were determined using 3-fold serial dilutions.The bacteriostatic kinetic curves of the 12 aquatic pathogens were determined.In addition,based on the potential biocompatibility of nano materials,we used MTT method to detect the cytotoxicity of V;-CDs on CIK cells.Danio rerio embryos were used as test objects to determine the embryotoxicity after exposure to V;-CDs.The results showed that V;-CDs had significant inhibitory effect on 12 aquatic pathogenic bacteria,and the minimum inhibitory concentrations ranged from 20.6 to 61.7μg/mL,basically reaching the same antibacterial effect as the antibiotic florfenicol.The survival rate of zebrafish embryos and CIK cells was almost 100%in the range of MIC.This study shows that V;-CDs have good bactericidal and antibacterial effects on the main pathogenic bacteria in aquaculture,and have good biocompatibility,which signifies the potential as a promising alternative to antibiotics in the prevention and control of bacterial diseases in aquaculture.

关 键 词：水产病原菌 维生素C碳点 抗菌性能 生物相容性 

分 类 号：S942.2[农业科学—水产养殖]