超声联合4-羟苯基维胺微泡对瘢痕疙瘩成纤维细胞Ⅰ型胶原蛋白α1链表达的影响  

Effect of ultrasound combined with 4-hydroxyphenyl-retinamide lipid microbubbles on typeⅠcollagenα1 chain expression in keloid-derived fibroblasts

作　　者：李周娜 金玟言 金哲虎 Li Zhouna;Jin Wenyan;Jin Zhehu(Department of Dermatology,Yanbian University Hospital,Yanji 133000,Jilin,China)

机构地区：[1]延边大学附属医院皮肤科,延吉133000

出　　处：《中华皮肤科杂志》2022年第7期596-598,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金项目(81960561);吉林省教育厅科研项目(JJKH20191148KJ);延边大学科研项目(延大科合字(2018)38号)。

摘　　要：目的探讨超声联合4-羟苯基维胺(4-HPR)微泡对瘢痕疙瘩成纤维细胞(KF)Ⅰ型胶原蛋白α1链(COL1A1)表达的影响。方法体外培养KF,分为对照组、15 mg/L 4-HPR微泡组和超声联合15 mg/L 4-HPR微泡组,处理24 h后,运用逆转录PCR(RT-PCR)和Western印迹法分别测定各组KF中COL1A1 mRNA及其蛋白的表达。结果对照组、4-HPR微泡组和超声联合4-HPR微泡组COL1A1 mRNA相对表达量分别为1.00±0.18、0.69±0.15和0.35±0.18,COL1A1蛋白相对表达水平为0.93±0.03、0.74±0.07和0.44±0.06。4-HPR微泡组和超声联合4-HPR微泡组COL1A1 mRNA和蛋白表达水平均明显低于对照组,差异均有统计学意义(P<0.05或0.001);而超声联合4-HPR微泡组COL1A1 mRNA和蛋白表达水平均显著低于4-HPR微泡组(均P<0.05)。结论超声联合4-HPR微泡对KF中COL1A1 mRNA及蛋白的表达有明显抑制作用。Objective To investigate the effect of ultrasound combined with 4-hydroxyphenyl-retinamide(4-HPR)lipid microbubbles on typeⅠcollagenα1 chain(COL1A1)protein expression in keloid-derived fibroblasts.Methods In vitro cultured keloid-derived fibroblasts were divided into 3 groups:control group receiving conventional culture with incomplete Dulbecco′s modified Eagle′s medium(DMEM),4-HPR lipid microbubble group cultured with incomplete DMEM containing 15 mg/L 4-HPR lipid microbubbles,and ultrasound+4-HPR lipid microbubble group cultured with incomplete DMEM containing 15 mg/L 4-HPR lipid microbubbles under ultrasound treatment.After 24-hour treatment,reverse transcription(RT)-PCR and Western blot analysis were performed to determine the mRNA and protein expression of COL1A1 in keloid-derived fibroblasts in each group.Intergroup comparison was carried out by using t test.Results The mRNA relative expression level of COL1A1 was 1.00±0.18,0.69±0.15 and 0.35±0.18 in the control group,4-HPR lipid microbubble group and ultrasound+4-HPR lipid microbubble group respectively,and the protein relative expression level of COL1A1 was 0.93±0.03,0.74±0.07 and 0.44±0.06 in the above 3 groups respectively.Moreover,the mRNA and protein expression of COL1A1 was significantly lower in the 4-HPR lipid microbubble group and ultrasound+4-HPR lipid microbubble group than in the control group(P<0.05 or 0.001),and lower in the ultrasound+4-HPR lipid microbubble group than in the 4-HPR lipid microbubble group(P<0.05).Conclusion Ultrasound combined with 4-HPR lipid microbubbles could markedly inhibit the mRNA and protein expression of COL1A1 in keloid-derived fibroblasts.

关 键 词：瘢痕疙瘩 胶原Ⅰ型 维甲酸 超声处理 4-羟苯基维胺 Ⅰ型胶原蛋白α1链 微泡 

分 类 号：R445.1[医药卫生—影像医学与核医学] R622[医药卫生—诊断学]