一株辣椒疫霉拮抗菌Y4-39分离鉴定及生防作用研究  

Isolation and Identification of an Antagonistic Strain Y4-39 and Its Application for Biocontrolling Phytophthora capsici

作　　者：邹修为 熊有明 谢剑波 肖志鹏 颜瑾[1] 陈武[1] 唐前君[1] 王运生[1] ZOU Xiu-wei;XIONG You-ming;XIE Jian-bo;XIAO Zhi-peng;YAN Jin;CHEN Wu;TANG Qian-jun;WANG Yun-sheng(College of Plant Protection,Hunan Agricultural University,Changsha 410128,PRC;Hengyang City Company,Hunan Provincial Tobacco Corporation,Hengyang 421001,PRC;Agricultural and Rural Bureau of Xinshao County,Xinshao 422900,PRC)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]衡阳市烟草公司,湖南衡阳421001 [3]邵阳市新邵县农业农村局,湖南新邵422900

出　　处：《湖南农业科学》2022年第6期1-5,共5页Hunan Agricultural Sciences

基　　金：湖南省自然科学基金(2020JJ4361)。

摘　　要：用稀释涂布法从黑水虻幼虫肠道中分离菌株,采用平板对峙法进行拮抗菌筛选,获得了1株对辣椒疫霉菌具有高效抑制作用的菌株Y4-39,经形态学、生理生化和16S rDNA序列分析,鉴定其为贝莱斯芽孢杆菌(Bacillus velezensis);其抑菌活性物质主要为抗菌肽类物质,能经受80℃以下高温处理,但对蛋白酶和紫外线较敏感;盆栽试验结果表明,菌株Y4-39发酵液对辣椒疫霉具有良好的防治效果,20 mL/株发酵液灌根处理对辣椒疫霉的防效达67.78%。该菌株能在黑水虻幼虫肠道中存活并大量繁殖,与虫沙一起可开发为具有生防功能的生物菌肥。There are lots of microbes in the intestine of black soldier fly(Hermetia illucens),and many of them have antimicrobial activity.Using the diluted method,we made strain isolation from the gut of the larvae and then obtained an antagonistic strain Y4-39 with high inhibitory activity on Phytophthora capsici by the plate confrontation method.Strain Y4-39 was identified as Bacillus velezensis based on the morphology,biochemical and physiological tests and 16S rDNA sequences analysis.The antimicrobial substances produced by Y4-39 were mainly antibacterial peptides that could retain activity under temperatures less than 80℃,but were sensitive to protease and violet ray treatment.The control efficacy for Phytophthora capsici using 20 mL Y4-39 broth per plant by root-drenching method could reach 67.78%in the pot experiment.Strain Y4-39 can survive and proliferate in the intestine of Hermetia illucens larvae,which has shown a potential biocontrol ability to act as bio-fertilizer agent with insect feces.

关 键 词：黑水虻 肠道微生物 辣椒疫霉拮抗菌 生物菌肥 

分 类 号：Q939.98[生物学—微生物学]