中药药效物质研究方法及进展  

Research methods and progress of effective components in traditional Chinese medicines

作　　者：乔雪[1] 张亚群 果德安 叶敏[1] QIAO Xue;ZHANG YaQun;GUO DeAn;YE Min(State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China;National Engineering Laboratory for TCM Standardization Technology,Shanghai Research Center for Modernization of Traditional Chinese Medicine,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China)

机构地区：[1]北京大学药学院,天然药物及仿生药物国家重点实验室,北京100191 [2]中国科学院上海药物研究所,上海中药现代化研究中心,中药标准化技术国家工程实验室,上海201203

出　　处：《中国科学：生命科学》2022年第6期908-919,共12页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:81725023,81891010/81891011,82122073);国家重点研发计划(批准号:2017YFC1700405)资助。

摘　　要：中药药效物质是指表征中药疗效的活性成分,阐明中药的药效物质是中医药现代化研究的重要内容.由于中药“多成分、多靶点”的特性,其药效物质研究具有较大的挑战性,表现为化学成分与体内代谢产物的全面准确定性定量分析存在困难,以及药效成分发现的效率不高、不够全面.近几十年,我国学者通过引入最新的仪器分析技术、细胞与分子生物学技术及计算机技术,创新性地探索建立了一系列富有中药特色的研究思路与方法.本文对这些研究方法及其进展进行了综述,包括中药化学成分分析新方法、中药体内代谢产物分析、中药药效物质发现三个方面,并对未来研究方向进行了展望.The bioactive constituents responsible for the therapeutic effects of traditional Chinese medicines(TCMs)are called effective components.Elucidating the effective components is a crucial part of TCM modernization research.Because of the“multicomponent and multitarget”features of TCMs,it is challenging to reveal their effective components,particularly to analyze the chemical constituents and in vivo metabolites globally and to discover the bioactive compounds efficiently.In recent decades,Chinese scientists have innovatively explored and established a series of new research strategies and methodologies for TCM effective components.State-of-the-art techniques in analytical chemistry,cell and molecular biology,and computer science have been employed.In this review article,we summarized these methodologies and recent research progress.The review includes three parts:new methodologies for chemical analysis,in vivo metabolites analysis,and effective components discovery.Future research perspectives are also discussed.

关 键 词：中药 药效物质 多成分、多靶点 中药分析 中药代谢 

分 类 号：R285[医药卫生—中药学]