哈尔滨地区猫白血病病毒流行情况及遗传变异分析  

Epidemic situation and genetic variation analysis of Feline leukemia virus in Harbin area

作　　者：马丽莹 刘恩琪 黄舒萍 尤丹 郭立君 郜旭 王亚君[1] MA Liying;LIU Enqi;HUANG Shuping;YOU Dan;GUO Lijun;GAO Xu;WANG Yajun(College of Wildlife and Protected Area,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040

出　　处：《黑龙江畜牧兽医》2022年第10期86-89,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家林草局野生动物疫病监测与预警系统维护项目“哈尔滨地区流浪猫和圈养东北虎猫艾滋病病毒感染情况调查”。

摘　　要：为了了解哈尔滨地区猫白血病的发病情况及猫白血病病毒的遗传变异情况,试验收集了哈尔滨地区51份猫的血液样本,用PCR方法扩增该病毒保守区域pol基因,并对阳性样本进行同源性分析与遗传进化分析。结果表明:25份样本检测结果为阳性,阳性率为49%。其中,公猫样本17份,母猫样本8份;<2岁猫样本有20份,2~4岁猫样本有3份,>10岁猫样本有2份,体重<3 kg猫样本有6份,3~6 kg猫样本有15份,>5 kg猫样本有4份。25份样本的核苷酸序列与参考序列的核苷酸相似性为89.2%~100%。遗传进化分析显示它们来自同一个分支,遗传关系较近,可能与地理分布有关。说明哈尔滨地区的猫白血病的发病率较高并且病毒基因型高度相似。In order to understand the incidence of feline leukemia in Harbin area and the genetic variation of the virus,the experiment collected 51 blood samples from cats in Harbin area,PCR was used to amplify the pol gene in the conserved region of the virus,and the positive samples were analyzed for homology analysis and phylogenetic analysis.The results showed that 25 samples were tested positive,with a positive rate of 49%.Among them,there were 17 male cat samples and 8 female cat samples;20 cat samples were 0-2 years old,3 cat samples were 2-4 years old,and 2 cat samples were>10 years old;6 cat samples were less than 3 kg,15 cat samples were 3-6 kg,and 4 cat samples were>5 kg.The nucleotide similarity between the nucleotide sequences of the 25 samples and the reference sequence was 89.2%-100%.Genetic evolu⁃tion analysis showed that they came from the same branch,and the genetic relationship was relatively close,which may be related to the geo⁃graphical distribution.The results suggested that the incidence of feline leukemia in Harbin area was high and the virus genotypes were highly similar.

关 键 词：猫 白血病病毒 聚合酶链式反应 同源性 遗传进化分析 

分 类 号：S855.3[农业科学—临床兽医学] Q78[农业科学—兽医学]