黄芪多糖对玉米赤霉烯酮诱导鸡胸腺细胞凋亡的保护作用  

Protective effect of Astragalus polysaccharides on Zearalenone-induced apoptosis of chicken thymocytes

作　　者：胡会 许泽锴 张凯照 卢春亭 周伽羽 崔红杰 黄小红[1,2,3] HU Hui;XU Zekai;ZHANG Kaizhao;LU Chunting;ZHOU Jiayu;CUI Hongjie;HUANG Xiaohong(College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial University Key Laboratory of Integrated Chinese Traditional and Western Veterinary Medicine and Animal Health,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院,福州350002 [2]福建农林大学中西兽医结合与动物保健福建省高校重点实验室,福州350002 [3]福建农林大学福建省兽医中药与动物保健重点实验室,福州350002

出　　处：《黑龙江畜牧兽医》2022年第10期109-114,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：福建省中青年教师教育科研项目(JAT200108);福建农林大学科技创新专项(CXZX2020066A,CXZX2019072S)。

摘　　要：为了探讨黄芪多糖(Astragalus polysaccharides,APS)对玉米赤霉烯酮(Zearalenone,ZEA)诱导的鸡胸腺细胞凋亡的影响,试验以鸡原代胸腺细胞为材料,采用MTT法检测APS对鸡胸腺细胞的安全浓度;选取50,100,200,400μg/mL APS分别与ZEA同时处理胸腺细胞48 h,并设置细胞对照和ZEA模型对照,MTT法检测细胞存活率;应用实时荧光定量PCR法检测内质网应激和细胞凋亡相关基因mRNA的表达。结果表明:APS对鸡胸腺细胞的安全浓度为50~400μg/mL;与细胞对照比较,ZEA模型对照能极显著降低细胞存活率(P<0.01),且GRP78、ATF6、ATF4、Caspase-3、Bax基因mRNA相对表达量极显著升高(P<0.01),Bcl-2基因mRNA相对表达量极显著下降(P<0.01);与ZEA模型对照比较,不同浓度APS+ZEA能显著或极显著提高细胞存活率(P<0.05或P<0.01),且GRP78、ATF6、ATF4、Caspase-3、Bax基因mRNA相对表达量极显著下降(P<0.01),Bcl-2基因mRNA相对表达量极显著升高(P<0.01)。说明APS可通过抑制ZEA诱导的细胞凋亡,恢复胸腺细胞的活性,起到保护作用。In order to explore the effect of Astragalus polysaccharides(APS)on Zearalenone(ZEA)-induced apoptosis of chicken thymocytes,chicken primary thymocytes were used as materials,and the safe concentration of APS on chicken thymocytes was detected by MTT method.Thymocytes were treated with 50,100,200 and 400μg/mL APS and ZEA for 48 h,respectively,and the cell control group and ZEA model control were set,and the cell viability was detected by MTT method.RT-qPCR method was used to detect the mRNA expression of endoplasmic reticulum stress and apoptosis-related genes.The results showed that the safe concentrations of APS on chicken thymocytes were 50-400μg/mL.Compared with the cell control,the ZEA model control could significantly reduce the cell survival rate(P<0.01),and the rela⁃tive expressions of GRP78,ATF6,ATF4,Caspase-3 and Bax gene mRNA were significantly up-regulated(P<0.01).The relative expression of Bcl-2 gene mRNA was significantly down-regulated(P<0.01).Compared with the ZEA model control,different concentrations of APS+ZEA could significantly or extremely significantly improve the cell survival rate(P<0.05 or P<0.01),and the relative expression of GRP78,ATF6,ATF4,Caspase-3,Bax gene mRNA was extremely significantly down-regulated(P<0.01);the relative expression of Bcl-2 gene mR⁃NA was extremely significantly up-regulated(P<0.01).The results suggested that APS could play a protective role by inhibiting ZEA-induced apoptosis and restoring the activity of thymocytes.

关 键 词：黄芪多糖 玉米赤霉烯酮 鸡胸腺细胞 内质网应激 细胞凋亡 

分 类 号：S859.3[农业科学—临床兽医学]