基于大黄酸碳点荧光探针检测烟叶含水量的研究  

Detection of Moisture Content in Tobacco Based on Rhein Carbon Dot Fluorescent Probe

作　　者：沈劭怡 李斌 杨德志 杨亚玲[2] SHEN Shao-yi;LI Bin;YANG De-zhi;YANG Ya-ling(Tobacco Leaf Production Center,Yunnan Tobacco Leaf CO.,LTD,Kunming 650214,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南省烟草烟叶公司烟叶生产中心,云南昆明650214 [2]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《分析测试学报》2022年第7期1045-1051,共7页Journal of Instrumental Analysis

基　　金：昆明理工大学高层次人才研究平台建设经费(20200097)。

摘　　要：以Mg^(2+)和大黄酸作为前驱材料制备大黄酸碳点,基于水对大黄酸碳点荧光信号的猝灭作用,借助智能手机RGB信号采集技术,建立了简单快速测定烟叶加工过程中水含量的分析方法。荧光猝灭机制表明:质子溶剂水与非质子溶剂二甲基亚砜(DMSO)间的相互作用,可显著猝灭碳点的荧光信号。在30~1000 mg范围内,水含量与荧光猝灭效果具有良好的线性关系,检出限达5 mg。利用智能手机采集信号,通过软件读取RGB值,结果表明在线性范围内水含量与R值有良好的线性关系(r^(2)=0.9902)。该方法可实现烟叶加工过程中水含量的现场快速测定,结果满足实际需求。方法具有简便、稳定、准确等特点,为水含量测试技术和相关探针设计提供了新的思路。Mg^(2+) and rhein were used as precursor materials to prepare rhein-carbon dots.Based on the quenching effect of moisture on the fluorescence signal of rhein-carbon dots,a simple and rapid method for the determination of moisture content in tobacco leaf processing was established with the help of smartphone RGB signal acquisition technique.The fluorescence quenching mechanism showed that the interaction between the protic solvent(water)and the aprotic solvent(DMSO)could significantly quench the fluorescence signal of rhein-carbon dots.There was a good linear relationship between fluorescence quenching effect and the water content in the range of 30-1000 mg,with a detection limit of 5 mg.Using a smartphone to collect signals and reading RGB values through software,the results showed that there was a good linear relationship between the water content and the R value within the linear range(r^(2)=0.9902).The method could realize rapid on-site determination of water content in tobacco leaf processing,and the results met the actual determination requirements.With the characteristics of simplicity,stability and accuracy,this method provides a new research strategy for water detection techniques and related probe design.

关 键 词：碳点 水分测定 大黄酸 智能手机 比色法 荧光探针 

分 类 号：O657.3[理学—分析化学] TS41.2[理学—化学]