大额牛瘤胃细菌CDT-1蛋白的生物信息学特征研究  

Bioinformatics characterization of rumen bacteria CDT-1 protein of Bas frontalis

作　　者：周子健 吴东旺 张光荣 和虎生 杨舒黎[1,2] ZHOU Zijian;WU Dongwang;ZHANG Guangrong;HE Husheng;YANG Shuli(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science,Kunming 650201,China;Agriculture and Rural Bureau of Gongshan Dulong and Nu Autonomous County,Nujiang Prefecture,Gongshan Dulong and Nu Autonomous,County 673599,China)

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]云南省动物营养与饲料科学重点实验室,昆明650201 [3]怒江州贡山独龙族怒族自治县农业农村局,云南贡山独龙族怒族自治县673599

出　　处：《黑龙江畜牧兽医》2022年第9期6-10,138,139,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(32060762);云南省农业基础联合专项(2017FG001〔-061〕)。

摘　　要：为了研究从云南大额牛(Bas frontalis)瘤胃微生物宏基因组文库中发掘出的纤维糊精酶-1(CDT-1)蛋白的生物信息学特征,试验使用ProtParam、TMHMM Server 2.0、ProtScale、NCBI Conserved Domains、SignalP 5.0、TargetP 2.0、PSORTⅡserver、SOPMA、SWISS-MODEL、NetN Glyc 1.0 Server、NetP hos 3.1 Server、NCBI protein BLASTp和MEGA 7.0等在线分析工具和软件,分析CDT-1蛋白的基本理化性质、跨膜区、亲水性/疏水性、结构域、信号肽、亚细胞定位、二级结构、三级结构、N端糖基化位点和丝氨酸、苏氨酸和酪氨酸的磷酸化位点及系统进化情况等生物信息学特征。结果表明:CDT-1蛋白的相对分子质量为38082.24,理论等电点为4.80,带负电荷的残基总数为45个,带正电荷的残基总数为29个,估计半衰期为30 h,属于亲水型稳定的脂蛋白,定位于细胞壁和细胞外,含有1个由α-螺旋、无规则卷曲、扩展链和β-转角组成的保守结构域,三级结构同源建模有较高的可信度,有2个N端糖基化位点和26个磷酸化位点,可能具有较高的酶活力;CDT-1蛋白与系统进化树中的内切葡聚糖酶的纤维素酶蛋白高度同源。说明CDT-1蛋白是来源于内切葡聚糖酶中的纤维素酶蛋白的亲水型稳定脂蛋白。In order to study the bioinformatics characteristics of cellodextrinase-1(CDT-1)protein excavated from Rumen Microbial Metagenomic Library of Yunnan Gayal(Bos frontalis),ProtParam,TMHMM Server 2.0,ProtScale,NCBI Conserved Domains,SignalP 5.0,TargetP 2.0,PSORTⅡserver,SOPMA,SWISS-MODEL,NetN Glyc 1.0 Server,NetP hos 3.1 Server,NCBI protein BLASTp and MEGA 7.0 and other online analysis tools and software were used to analyze the basic physical and chemical properties,transmembrane region,hydrophilicity/hydrophobicity,protein domain,signal peptide,subcellular localization,secondary structure,tertiary structure,N-terminal glycosylation site,protein serine,threonine and tyrosine phosphorylation sites and system evolution of CDT-1 protein.The results showed that the relative molecular mass of CDT-1 protein was 38082.24;the theoretical isoelectric point was 4.80;the total number of negatively charged residues was 45;the total number of positively charged residues was 29;and the estimated half-life was 30 h;and the water-type stable lipoprotein was located in extracellular with cell wall and contained a conserved domain composed ofα-helix,random coils,extended strands,andβ-turns.The tertiary structure homology modeling had a high degree of credibility.It had two N-terminal glycosylation sites and 26 phosphorylation sites,which might have high enzyme activity.CDT-1 protein was highly homologous to the cellulase protein of endoglucanase in the phylogenetic tree.It indicated that the CDT-1 protein was a hydrophilic stable lipoprotein derived from cellulase protein in endoglucanase.

关 键 词：大额牛 CDT-1蛋白 生物信息学 蛋白质功能 瘤胃细菌 

分 类 号：S823[农业科学—畜牧学] Q349.53[农业科学—畜牧兽医]