鸡IL-4和IL-2重组融合基因佐剂对鸡球虫活疫苗的增效作用研究  

Synergistic effect of recombinant fusion gene adjuvant of chIL-4 and chIL-2 on chicken coccidiosis live vaccine

作　　者：崔凯玲 郝飞飞 郑明学[1] 吕晓玲 贡鑫 郑龙龙 徐彤[1] 白瑞[1] CUI Kailing;HAO Feifei;ZHENG Mingxue;LYU Xiaoling;GONG Xin;ZHENG Longlong;XU Tong;BAI Rui(College of Animal Medicine,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西农业大学动物医学学院,山西晋中030801

出　　处：《黑龙江畜牧兽医》2022年第9期18-22,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31972647);山西省青年科技研究项目(201901D211373);山西农业大学科技创新项目(2020BQ08);山西省优秀博士科研奖励计划项目(SXYBKY2019023)。

摘　　要：为了研究鸡白细胞介素4(IL-4)和IL-2重组基因佐剂pCI-chIL-4-chIL-2-EGFP对鸡球虫活疫苗的增效作用,试验采用基因佐剂配合鸡球虫活疫苗对雏鸡进行免疫攻毒试验,测定该基因佐剂的最小有效剂量、给药途径,使用该基因佐剂对鸡球虫活疫苗的最短免疫间隔时间和免疫产生期的影响。结果表明:抗球虫指数(ACI)和相对增重率(RWG)随着基因佐剂剂量增高而升高,肌肉注射100μg/只时,ACI(189.32)和RWG(90.35%)达到高峰;口服基因佐剂300μg/只组的ACI(188.41)与肌肉注射基因佐剂100μg/只组接近;肌肉注射佐剂免疫攻毒组的两次免疫最短间隔时间(6 d)较不加基因佐剂组(7 d)缩短1 d;肌肉注射佐剂免疫攻毒组的免疫产生期为二免后第5天,较无基因佐剂免疫攻毒组提前1 d。说明肌肉注射IL-4和IL-2重组基因佐剂pCI-chIL-4-chIL-2-EGFP 100μg/只可提高鸡球虫活疫苗的免疫效果,缩短免疫产生期和免疫间隔。In order to study the synergistic effect of chicken interleukin-4(IL-4)and IL-2 recombinant gene adjuvant pCI-chIL-4-chIL-2-EGFP on chicken coccidiosis live vaccine,the immune challenge test was carried out by combining genetic adjuvant with chicken coccidiosis live vaccine.The minimum effective dose and route of administration of the gene adjuvant were determined,and the effects of the gene adjuvant on the shortest immune interval time and immune generation period of chicken coccidiosis live vaccine were determined.The results showed that anticoccidial index(ACI)and relative weight gain rate(RWG)increased with the increase of the dose of gene adjuvant,and the ACI(189.32)and RWG(90.35%)reached the peak when the dose of gene adjuvant was at 100μg/chinken.The ACI(188.41)of the oral 300μg/gene adjuvant group was close to that of the intramuscular 100μg/gene adjuvant group.The shortest time between two immunizations(6 d)in gene adjuvant challenge group(100μg/chinken)was shorter by 1 d than that in gene adjuvant challenge group(7 d).The immune generation period of the intramuscular adjuvant immune challenge group was 5 days after the second immunization,1 d earlier than that of the non-gene adjuvant immune challenge group.These results indicated that intramuscular injection of 100μg/chinken with pCI-chil-4-Chil-2-EGFP,IL-4 and IL-2 recombinant gene adjuvant could improve the immune effect of chicken coccidiosis live vaccine and shorten the immune generation period and interval.

关 键 词：基因佐剂 鸡球虫活疫苗 白细胞介素4(IL-4) IL-2 剂量 免疫效果 

分 类 号：S852.72[农业科学—基础兽医学]