阳原驴MSTN基因5'UTR多态性分析  

Polymorphism analysis of 5'UTR region of MSTN gene in Yangyuan donkeys

作　　者：付永智 马晓君 陈芝媛 王茂晗 赵涵 李哲 苏咏梅[1] 朱文进[1] FU Yongzhi;MA Xiaojun;CHEN Zhiyuan;WANG Maohan;ZHAO Han;LI Zhe;SU Yongmei;ZHU Wenjin(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China)

机构地区：[1]河北科技师范学院动物科技学院,河北秦皇岛066004

出　　处：《黑龙江畜牧兽医》2022年第9期132-135,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：河北省重点研发计划项目(19226379D);河北省高等学校科学技术研究项目(ZD2014010,ZD2015117);河北科技师范学院动物科技学院院级科研项目(DK2020-10)。

摘　　要：为探究MSTN基因5'UTR多态性及其对阳原驴体尺指标(体高、体长、胸围)的影响,试验测量了舍饲的70头2岁以上阳原驴的体尺指标,用PCR-SSCP技术进行MSTN基因5'UTR多态位点检测,并对不同基因型个体的体尺性状进行差异分析。结果表明:阳原驴MSTN基因5'UTR存在g.229T>C突变,产生TT、CC和TC三种基因型,不同基因型个体之间的体高、体长差异不显著(P>0.05),而CC基因型个体胸围显著大于其他两个基因型(P<0.05)。说明第229位点可以作为阳原驴选择育种的候选标记。In order to investigate the polymorphism of MSTN gene in the 5'UTR region and its effect on body size indexes(body height,body length and chest circumference)of Yangyuan donkeys,70 yangyuan donkeys over 2 years old were measured.PCR-SSCP technique was used to detect the polymorphic loci in the 5'UTR region of MSTN gene,and the differences of body size traits among different genotypes were analyzed.The results showed that there was g.229T>C mutation in the 5'UTR region of MSTN gene of Yangyuan donkeys,which produced TT,CC and TC genotypes.There were no significant differences in body height and body length among different genotypes(P>0.05),but chest circumference of CC genotype was significantly larger than that of the other two genotypes(P<0.05).The results indicated that site 229 could be a candidate marker for selection and breeding of Yangyuan donkeys.

关 键 词：阳原驴 MSTN基因 5'UTR SNPS 体尺性状 

分 类 号：S822[农业科学—畜牧学] Q754[农业科学—畜牧兽医]