套袋对海沃德猕猴桃果实品质及叶绿素代谢的影响  

Effect of bagging on fruit quality and chlorophyll metabolism of Hayward kiwifruit

作　　者：陈成 王依 宋思言 杨勇[1] 万春雁[1] 阎永齐[1] CHEN Cheng;WANG Yi;SONG Siyan;YANG Yong;WAN Chunyan;YAN Yongqi(Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province,Jurong,Jiangsu 212400,China;Yanliang Agricultural Technology Spreading Center,Xi’an,Shaanxi 710089,China)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400 [2]西安市阎良区农业技术推广中心,陕西西安710089

出　　处：《西北农林科技大学学报（自然科学版）》2022年第7期138-146,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：江苏省农业科技自主创新资金项目(CX(20)3188)。

摘　　要：【目的】探究套袋对海沃德猕猴桃果实品质、色泽及叶绿素代谢等指标的影响,为猕猴桃叶绿素代谢机理研究提供科学依据。【方法】于盛花期后30 d,以不套袋作为对照(CK),用外黄内黑单层袋、外黄内黑双层袋、白色单层袋、黄色条纹单层袋4种类型果袋对海沃德猕猴桃果实进行套袋处理,测定并分析不同处理猕猴桃果实外观品质(果实纵径、横径、侧径及单果质量)、内在品质(可溶性固形物含量、总酸含量、固酸比、果实硬度及干物质含量)、糖含量(果实蔗糖、果糖及葡萄糖含量)、果皮与果肉色泽变化、果实叶绿素含量及叶绿素代谢相关基因的表达情况。【结果】与对照相比,各套袋处理对海沃德猕猴桃果实纵径、横径、侧径、单果质量、可溶性固形物含量及干物质含量均无显著影响;除白色单层袋处理外,其余套袋处理果实总酸含量均有所下降,固酸比均有所上升。各套袋处理果实蔗糖含量均显著降低,最多较对照降低66.50%;除外黄内黑双层袋处理外,其余套袋处理果实果糖含量和葡萄糖含量均有所下降。外黄内黑单层袋和外黄内黑双层袋处理果皮a值、果肉a和h值均显著增加,果肉b、C^(*)、h^(*)值均显著降低;套袋处理果实叶绿素a及总叶绿素含量均显著降低,最大降幅分别为93.20%和91.60%。实时荧光定量PCR分析发现,套袋处理后果实叶绿素合成相关基因AdCAO表达丰度显著下调,AdGLUTR3表达丰度总体呈下调趋势;黄色条纹单层袋处理后叶绿素降解相关基因AdCBR1、AdPPH2表达丰度显著上调,AdCBR2、AdCBR3、AdPAO、AdSGR1、AdPPH1、AdPPH3表达丰度均有所上调。【结论】套袋处理对猕猴桃果实外观品质并无显著影响,但会改变果实糖组分所占比例;同时,果实叶绿素合成及降解相关基因的表达丰度发生改变,叶绿素含量降低,果肉颜色发生改变。【Objective】The effects of bagging on fruit quality,color and chlorophyll metabolism of Hayward kiwifruit were explored to provide basis for the study of chlorophyll metabolism.【Method】Fruit bagging treatments were performed with four kinds of fruit bags including outer yellow and inner black single-layer bag,outer yellow and inner black double-layer bag,white single-layer bag and yellow stripes single-layer bag around 30 d after full-bloom flowering with no bagging as the control(CK).Fruit appearance quality(longitudinal diameter,transverse diameter,lateral diameter and weight),fruit inherent quality(soluble solid content,total acid content,SSC/TAC,fruit firmness and dry matter content),sugars content(sucrose,fructose and glucose),color changes of epicarp and flesh,chlorophyll concentration and expression patterns of chlorophyll-related genes were determined and analyzed.【Result】Compared with CK,different bagging treatments had no significant effects on the longitudinal diameter,transverse diameter,late-ral diameter,fruit weight,soluble solid content and dry matter content.Except white single-layer bag treatment,the total acid content decreased and solid-acid ratio increased in other treatments.The sucrose content of kiwifruit decreased significantly by bagging treatments with the most reduction of 66.50%.Except outer yellow and inner black double-layer bag,the fructose and glucose concentrations of fruit decreased in other bagging treatments.In treatments of outer yellow and inner black single-layer bag and outer yellow and inner black double-layer bag,a value of epicarp increased significantly,a and h values of flesh increased significantly,while b,C^(*) and h^(*) values of flesh decreased significantly.Meanwhile,chlorophyll a and total chlorophyll concentrations of flesh were decreased significantly by bagging treatments with the maximum reductions of 93.20%and 91.60%,respectively.Real-time quantitative PCR showed that the expression abundance of chlorophyll biosynthetic gene AdCAO was significantl

关 键 词：猕猴桃 套袋 果实品质 叶绿素代谢 

分 类 号：S663.405.9[农业科学—果树学]