杨梅素类衍生物H10对肺癌细胞H460影响的体外实验研究  

Experimental study of myricetin derivative H10 on lung cancer in vitro

作　　者：李艳[1] 刘静[1] 刘健[1] LI Yan(Department of gynecology and oncology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,China)

机构地区：[1]蚌埠医学院第一附属医院妇瘤科,安徽蚌埠233000

出　　处：《牡丹江医学院学报》2022年第4期40-43,78,共5页Journal of Mudanjiang Medical University

基　　金：安徽省教育厅重点课题(kj2019A0363)。

摘　　要：目的研究杨梅素衍生物H10对肺癌细胞H460影响。方法通过四甲基偶氮唑盐法(MTT比色法)法研究H10对H460细胞的增殖影响,通过细胞成像法和DAPI-Actin染色观察H10处理后细胞形态学的变化及细胞凋亡情况,细胞克隆形成实验检验H10对细胞间依附成团及增殖能力影响,伤口愈合实验研究H10是否抑制H460细胞的迁移能力,流式分析H10诱导肺癌细胞H460发生凋亡。结果MTT比色法表明H10具有浓度依赖性抑制肺癌细胞H460细胞的增殖能力,其中H10的IC50=1.412μM;细胞成像法和DAPI-Actin染色观察到随着H10药物浓度增加,H460细胞数目明显减少,细胞缩小,变圆变亮,周围出现细胞碎片;细胞克隆形成实验结果表明H10具有浓度依赖性抑制肺癌细胞H460细胞间粘附能力;伤口愈合实验及流式分析凋亡结果表明H10可抑制肺癌细胞迁移、诱导凋亡。结论杨梅素衍生物H10在肺癌细胞H460中展现出显著抑制细胞增殖、迁移及诱导细胞凋亡作用。Objective To investigate the effect of populistin derivative H10 on lung cancer cells H460.Methods The effect of H10 on the proliferation of H460 cells was detected by methyl thiazolyl tetrazolium method(MTT assay),the morphological changes and apoptosis of cells treated with H10 were observed by cell imaging method and DAPI-Actin staining,the cell clone formation assay was used to examine the effect of H10 on intercellular attachment into clusters and proliferation ability,the wound healing assay was used to investigate whether H10 inhibited the migration ability of H460 cells,and the flow analysis was used to detect the apoptosis of lung cancer cells H460.Results MTT assay showed that H10 had concentration-dependent inhibition of proliferation of lung cancer cells H460 cells,in which the IC50 of H10=1.412μM.Cell imaging and DAPI-Actin staining observed that with the increase of H10 drug concentration,the number of H460 cells decreased significantly,the cells shrunk,became round and bright,and cell fragments appeared around them.Cell clone formation assay showed that H10 inhibited the intercellular adhesion of H460 cells in a concentration-dependent manner.Wound healing assay and flow analysis of apoptosis showed that H10 inhibited the migration of lung cancer cells and induced apoptosis.Conclusion Myricetin derivative H10 exhibits significant inhibited effects on cell proliferation and migration,and induces the apoptosis of lung cancer cells H460.

关 键 词：杨梅素类衍生物H10 肺癌 增殖 迁移 

分 类 号：R285[医药卫生—中药学]